神經元 (neuron) 是一種高度特化細胞，具許多特化性次細胞結
構。分離純化次細胞結構進行蛋白質分析方面一直是科學家們有興趣
研究的課題。軸突是神經細胞中最長的神經纖維，主要功能為由細胞
本體與軸突尾端間傳遞動作電位、訊號及物質。就目前文獻中並無好
的工具方法能夠收集大量且單純的神經軸突蛋白質，因此我們發展一
套神經軸突收集裝置，此裝置可使神經元之細胞本體、樹突與軸突分
隔在兩個特定不同的環境，進而大量純化軸突。裝置包括上層由PCTE
（聚碳酸酯）孔徑為3 μm 的多孔細胞濾膜，和下層為具有孔洞且厚
度為300 μm 或500 μm 的PDMS 層組成。在上層PCTE 多孔細胞濾
膜表面生長的神經細胞的軸突與樹突可穿過細胞濾膜生長至下層具
有孔洞的PDMS 層洞孔中。樹突之平均長度較軸突短，一般僅有軸
突能長得夠長到達PDMS層之背面。本實驗目的想要測試厚度300 μm
或500 μm 的PDMS 層之背面是否能收集單純軸突。以此運用laminin
和collagen 之濃度梯度，引導海馬迴神經細胞之軸突往PDMS 層生
長。此裝置與最近本實驗室所發展一套神經細胞培養基板裝置目的相
同，透過此設計預期將可收集至20~30 倍的軸突產量，未來收集之軸
突將足以進行次細胞胞器及其結構之分離、研究、分析。

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