癌症一直以來都備受全球關注。儘管研究人員不斷努力尋找治療方法並持續進行發展，但仍然沒有完全根治癌症的方法。癌細胞轉移是使癌症難以治療的主要原因之一，其中腹膜轉移是最複雜以及最難處理的類型。腹膜轉移多發生在晚期癌症患者，涉及一種以上的癌細胞在患者的腹膜腔內擴散，主要源自胃癌、結直腸癌和卵巢癌等。為了治療這類患者，腹腔溫熱化療（Hyperthermic Intraperitoneal Chemotherapy, HIPEC）是一種新興的治療方法，通常在腹腔腫瘤減積手術（Cytoreductive Surgery, CRS）之後進行。手術過程中，溫度保持在42-43℃之間。同時，需要在腹腔內持續注入藥物60至90分鐘，利用熱和化療藥物來殺死癌細胞。
在本研究中，我們將開發一個藥物篩選的平台，使用微機電技術製造出晶片。在晶片中，藥物可以自動形成組合並直接流入腔室。此外，基於介電泳技術將癌細胞和纖維母細胞排列成特定的圖案並以特定比例組成，以模仿實際的腫瘤微環境。實驗過程中，將系統溫度保持在42～43℃，模擬出腹腔溫熱化學療法的治療過程。最後使用細胞死活螢光測定對細胞進行染色，以螢光共定位分析癌細胞之存活率。由實驗結果可以知道Cisplatin+Docetaxel的組合對癌細胞有最高的抑制效果，並且我們發現癌細胞會隨著纖維母細胞比例增加而有更強的抗藥性。

Cancer metastasis, particularly peritoneal metastasis, poses a significant challenge in cancer treatment. Despite ongoing research, no complete cure for cancer has been found. One emerging option for treating peritoneal metastasis is Hyperthermic Intraperitoneal Chemotherapy (HIPEC). HIPEC involves maintaining the surgical temperature at 42 to 43 ℃ while injecting chemotherapy drugs into the peritoneal cavity for 90 minutes. This combined approach utilizes heat and chemotherapy to eliminate cancer cells effectively.
This research aims to develop a drug screening platform for HIPEC. We employed the Microelectromechanical Systems (MEMS) technique to fabricate our microfluidic chips. This chip enables automatic drug combination formation and direct flow to the cell culture chamber. Moreover, we utilized the dielectrophoresis technique to arrange cancer cells and fibroblasts in specific patterns and compositions, mimicking the tumor microenvironment. Finally, we assessed cell viability using a fluorescence-based assay, analyzing cancer cell survival through colocalization analysis. The experimental results revealed that the combination of Cisplatin and Docetaxel exhibited the highest inhibitory effect on cancer cells. We also found that cancer cells exhibited increased resistance to the treatment with the escalation of fibroblast cell proportions.

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