細菌培養在微生物學上是個基本且重要的技術，而晶片上微小化生物反應室是為了改善大尺度生物反應室需要消耗大量的試劑與人力的問題，我們設計了一個兩間隔隔室的微流體生物反應室，並且利用它成功的完成了序列稀釋的實驗，我們的晶片尺度在奈升等級，利用電腦可自動化操作實驗並且長時間的觀測、培養微生物。由於晶片內的高面積體積比使得生物反應室不只可培養浮游生物，更能有效的成長生物膜，為了得到浮游微生物不受生物膜影響下的生長狀況，我利用介面活性劑Tween20抑制生物膜的成長並且量測出純浮游生物在反應室內的生長狀況。

Bacterial culture is a basic technique in both fundamental and applied microbiology.The excessive reagent consumption and laborious maintenance of bulk bioreactors for microbial culture have prompted the development of miniaturized on-chip bioreactors.With the minimal choice of two compartments (N=2) and discrete time,periodic dilution steps,we realize a microfluidic bioreactor that mimics macroscopic serial dilution transfer culture.This device supports automated, long-term microbial cultures with a nanoliter-scale working volume and real-time monitoring of microbial populations at single-cell resolution. The use of surfactant Tween20 suppresses the biofilm growth and enables the pure planktonic growth.

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