整合微製造技術與細胞培養，可將細胞生長作空間上的調控，以利基礎細胞生物學的研究。本論文利用梳狀結構引導神經突起向單一方向伸展，試圖將細胞體與神經突起做空間上的區隔，便於生長錐的收集。藉由光學微影與矽深蝕刻技術製作圖章與模板之模仁，以化學氣相沉積抗沾黏之聚對二甲苯(parylene)高分子膜，防止圖章組成材料與矽膜仁上的原生氧化層沾黏。彈性高分子材料─聚二甲基矽氧烷(PDMS)經鑄造、固化及脫模等步驟製造圖章與模板的PDMS高分子模仁，沉積parylene高分子膜於PDMS膜仁上，經由二次複製則可得到侷限細胞貼附區域的模板。以圖章為工具、微接觸印刷為方法，將輔助細胞貼附的生物分子─聚左旋離氨酸(PLL)轉印於基材上，或直接將模板覆蓋於經由PLL修飾的基材表面，藉由PLL所定義出的圖形或模板上的開孔限制細胞可貼附的範圍，同時引導神經突起的生長方向。在細胞培養後第二天即可觀察到神經細胞的散佈與成長已受PLL圖形的限制，細胞體與神經突起持續受到空間上的調控直到細胞死亡。然而，在培養過程中細胞體極容易聚集形成球狀，本文結合微接觸印刷與模板試圖解決聚集現象，由初步結果顯示未來可望藉此法維持細胞體正常貼附與引導神經突起生長。透過上述兩種微製造技術使神經細胞可生長成特定的陣列，以利後續生化分析實驗的進行。

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