鉑-磁鐵礦啞鈴型奈米粒子的製備、特性鑑定及在多巴胺檢測上的應用

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研究生：	廖祐祥 Liao, Yu-Hsiang
論文名稱：	鉑-磁鐵礦啞鈴型奈米粒子的製備、特性鑑定及在多巴胺檢測上的應用 Pt-Fe3O4 Dumbbell-like Nanoparticles: Synthesis, Characterization, and Application to Dopamine Detection
指導教授：	董瑞安 Doong, Ruey-an
口試委員:	孫毓璋吳希天
學位類別：	碩士 Master
系所名稱：	原子科學院 - 生醫工程與環境科學系 Department of Biomedical Engineering and Environmental Sciences
論文出版年：	2011
畢業學年度：	99
語文別：	中文
論文頁數：	82
中文關鍵詞：	多巴胺、生物感測器、啞鈴形奈米粒子
外文關鍵詞：	Dopamine, Biosensor, Dumbbell nanoparticle
相關次數：	點閱：2 下載：0
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　　本研究利用不同粒徑鉑奈米粒子結合磁鐵礦來製備鉑-磁鐵礦啞鈴型複合奈米粒子。利用Pt(acac)2作為鉑的前驅物以油胺作為還原劑，調控加熱溫度從140-180ºC及還原劑的劑量分別得到顆粒大小為3、6、13 nm的鉑奈米粒子。將Pt分散在Fe3O4的前驅物油酸鐵中，藉由調控油酸鐵及Pt的比例分別得到3、6、13 nm的Pt-Fe3O4啞鈴型奈米粒子。本研究同時也利用TEM、XRD、TGA、ICP-AES、SQUID對Pt及Pt-Fe3O4進行表面特性鑑定。電化學特性上，Pt-Fe3O4具有較佳電子轉移能力，將Pt及Pt-Fe3O4結合石墨電極組成安培式電化學感測器， 3、6、及13 nm Pt對多巴胺的線性範圍分別為20-850、15-850、35-850 µM，Pt-Fe3O4分別為15-850、20-850、10-850 □M，3、6、及13 nm Pt偵測極限分別為7.49、7.36、8 □M，Pt-Fe3O4分別為7.22、6.14、0.13 □M。Pt-Fe3O4表現出比Pt強的電流值訊號及相同的線性範圍並有較低之偵測極限，在應用上有較好的潛力。

　　In this study, the dumbbell-like Pt-Fe3O4 nanoparticle was prepared by using Pt nanoparticle with different sizes as the seeding nanoparticles. The 3, 6, and 13 nm of Pt nanoparticles were obtained by using Pt(acac)2 and oleylamine as the precursor and reducing agent, respectively. In addition, the different sizes of PT nanoparticles can be obtained by controlling the amount of reducing agent and synthesisis temperature from 140-180ºC. Using different sizes of Pt nanoparticles in the presence of iron-oleate, the different sizes of dumbbell-like Pt-Fe3O4 nanoparticle were successfully fabricated by tuning the ratios of Pt and iron-oleate. The synthesized Pt and Pt-Fe3O4 were characterized by TEM, XRD, TGA, ICP-AES, SQUID. The Pt showed better electron transfer ability. Pt and Pt-Fe3O4 were dispersed on graphite electrode to fabricate the amperomertic biosensor for sensing dopamine，the Pt-Fe3O4 electrode is linearly dependence on dopamine concentration in the range of 20-850,15-850 and 35-850 □M receptively , and the Pt-Fe3O4 electrode were 15-850,25-850,10-850 □M receptively. The detection limits of Pt were 7.49,7.36 and 8 □M receptively, and Pt-Fe3O4 were 7.22,6.14 and 0.13 □M receptively. In addition, the Pt-Fe3O4 shows better current value of signal intensity , linear range and more lower detection limits when compared Pt nanoparticles, clearly showing the application potential on biosensing and catalytic activity.

謝誌    II
中文摘要    III
Abstract    IV
第一章 前言--------------------------1
 1-1 研究動機------------------------1
 1-2 研究目的------------------------1
第二章 文獻回顧----------------------4
 2-1 奈米複合材料--------------------4
 2-1-1    核殼複合粒子----------------4
 2-1-2合金---------------------------5
 2-1-3    啞鈴型複合奈米粒子    ----------6
2-2    鉑奈米粒子------------------9
2-3    奈米材料的製備--------------11
 2-3-1    鉑奈米粒子的製備------------11
 2-3-2    啞鈴型奈米粒子的製備--------14
2-4    生物感測器(Biosensor)-------15
 2-4-1    光學生物感測器--------------16
 2-4-2    壓電晶體生物感測器----------18
  2-4-3    電化學生物感測器------------19
2-5    多巴胺----------------------22
 2-5-1    帕金森氏症------------------23
 2-5-2    多巴胺的檢測----------------24
第三章 材料與方法--------------------27
 3-1 試劑與材料----------------------28
 3-2 不同大小Pt奈米粒子之製備--------28
 3-3 Pt- Fe3O4奈米粒子之製備---------29
 3-3-1 油酸鐵之製備------------------29
 3-3-2 Pt- Fe3O4奈米粒子之製備-------30
 3-4  電極的製備---------------------30
 3-5 特性鑑定------------------------31
 3-5-1 穿透式電子顯微鏡--------------31
 3-5-2  X光繞射儀--------------------32
 3-5-3 熱重分析儀--------------------33
 3-5-4 感應耦合電漿發射光譜    ----------33
 3-5-5超導量子干涉儀-----------------33
 3-5-6 電化學特性分析----------------33
 3-6 多巴胺的檢測--------------------34
 3-6-1 安培式電化學感測器------------36
 3-6-2 偵測極限之測定----------------36
第四章 結果與討論--------------------37
 4-1 不同大小之Pt合成----------------37
 4-1-1 3nm Pt奈米粒子合成------------37
 4-1-2 6nm Pt奈米粒子合成------------38
 4-1-3 13nm Pt奈米粒子合成-----------40
 4-2 Pt- Fe3O4合成    -------------------42
 4-2-1 3nm Pt- Fe3O4的合成-----------42
 4-2-1 6nm Pt- Fe3O4的合成-----------44
 4-2-1 13 nm Pt-Fe3O4的合成----------44
 4-3 特性鑑定------------------------46
 4-3-1 X光繞射儀---------------------46
 4-3-2熱重分析及ICP-AES -------------49
 4-3-3 SQUID 分析--------------------51
 4-3-4 電化學特性測試----------------53
 4-4 多巴胺偵測----------------------61
第五章 結論--------------------------72
參考文獻-----------------------------74

                                

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