Introduction
The clinical and biological significance of human fungal pathogen Candida albicans has been markedly increased since the Candida infection can cause lethal systemic infections. However, the defensive and invasive mechanisms of host-pathogen interaction are not elucidated as a whole. Therefore, the crucial functional modules emerging in C. albicans-zebrafish interaction are investigated.
Results
From a systems biology perspective, the early-stage and late-stage protein interaction networks for both C. albicans and zebrafish were constructed by the database mining and simultaneous host-pathogen interaction time-profile microarray, respectively. Based on the network reconfiguration and the Gene Ontology database annotation, the critical functional modules were identified for offensive and defensive mechanisms in infection process. Hyphal morphogenesis, small molecule and ion transport, protein secretion, and shift of carbon utilization functional modules of C. albicans are highlighted to play important roles in invasion and damage on host cells. On the other hand, immune response, apoptosis mechanism, ion transport and hemostasis-related process functional modules of zebrafish are found to be significant for defense or survival during Candida infection.
Conclusion
The crucial functional modules investigated by both host and pathogen microarray data at early and late stage of infection gain more insights into the molecular mechanisms of host-pathogen interaction during infection process. With the understanding of host-pathogen interaction and the identification of significant reconfiguration proteins in those crucial functional modules, efficient therapeutic strategies can be provided to combat against Candida infection.

簡介
由於白色念珠菌可造成致命、全身性的感染，因此，此種人類真菌病源菌在臨床及生物上的重要性已明顯地增加。然而，尚未有從整體的角度來解釋宿主-病源菌交互作用之間的攻擊和防禦機制，因此進行白色念珠菌-斑馬魚交互作用中的重要功能性模組之研究探討。
結果
從系統生物學角度來看，藉由資料庫探勘及隨實驗時間點推移同步所得的宿主-病源菌交互作用之基因晶片實驗數據，分別替白色念珠菌和斑馬魚建立出感染前期和後期的蛋白質交互作用網路。根據網路重組和基因功能註解，可辨認出在感染過程中的攻擊性和防禦性的重要功能性模組。白色念珠菌於入侵和損害宿主細胞時，菌絲形態發生、小分子和離子運輸、蛋白質分泌、碳利用轉換之功能性模組，扮演著重要的角色。另一方面，斑馬魚的功能性模組：免疫、細胞凋零、離子運輸、止血相關過程，對於斑馬魚面對念珠菌感染時的防禦或生存而言是重要的。
結論
藉由感染前期和後期的宿主和病源菌的基因晶片實驗數據，重要功能性模組得以研究，且增加了對於感染過程中宿主-病源菌交互作用的分子機制的理解。透過了解宿主-病源菌的交互作用以及辨認出重要的功能性模組中的蛋白質，可藉此提供有效的治療策略來對抗因白色念珠菌感染所造成的疾病。

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