本論文之研究成果，是以豬的大腿關節軟骨為模型，建立一個在軟骨組織中直接萃取到最大量軟骨中蛋白質，並進行鑑定的方法學。在80℃下，經過冷凍乾燥的軟骨組織經過10分鐘的萃取，可達到最高的產率。再經過透析、氯仿-甲醇沈澱等步驟以後，可進一步進行二維電泳和MALDI-TOF等蛋白質體學的分析，一共有有17種蛋白質被鑑定出來。
經過Cetyl pyridinium chloride（CPC）沈澱可將從軟骨組織萃取出蛋白質中的proteoglycan分離，並改善第一維等電焦集電泳（IEF）中蛋白質分離的狀況。但這方法除了結果再現性不高以外，還會流失大量的蛋白質，造成所鑑定出來的蛋白質並不是完整的軟骨組織的蛋白質體（proteome）。
因此結果發現，我們的樣本萃取與處理方法可以鑑定出較完整的軟骨蛋白質體，往後可用於臨床上正常與病變軟骨組織上的分析，其後可建立一個軟骨組織的蛋白質體資料庫，並進行資料庫的比對，進一步可以找到病變軟骨的bio-protein marker。

C. Bassleer, P. Gysen, R. Bassleer and P. Franchimont. (1987) Effects of peptidic glycosaminoglycans complex on human condrocytes cultivated in three dimentions. Biochemical Pharmacology, 37 (10):1939-1945

Cristina Ruiz-Romero, Maria J. Lopez-Armada and Francisco J. Blanco. (2005) Proteomic characterization of human normal articular chondrocytes: A novel tool for the study of osteoarthritis and other rheumatic diseases. Proteomics, 5 (12): 3048–3059

Deere M, Sanford T, Francomano CA, Daniels K, Hecht JT. (1999) Identification of nine novel mutations in cartilage oligomeric matrix protein in patients with pseudoachondroplasia and multiple epiphyseal dysplasia. American Journal of Medical Ggenetics ,85 (5):486-90

Geng,Y.,Blanco,F.J.,Cornlisson M.,and Loyz M. (1995) Regulation of cyclooxygenase-2 expression in normal human articular chondrocyte. The Journal of Immunology 155, 796-801

Helen Muir. (1995) The chondrocyte,architect of cartilage. Bio Eaasys 17 (12): 1039-1048

Hiroshi Yamagiwa, Gobinda Sarkar, M. Cristine Charlesworth, Daniel J. McCormick, and Mark E. Bolander1 (2003) Two-dimensional gel electrophoresis of synovial fluid: method for detecting candidate protein markers for osteoarthritis. Journal of Orthopaedic Science 8:482–490

J. B. Catterall, A. D. Rowan, S. Sarsfield, J. Saklatvala, R. Wait1 and T. E. Cawston. (2006) Development of a novel 2D proteomics approach for the identification of proteins secreted by primary chondrocytes after stimulation by IL-1 and oncostatin M. Rheumatology 10:1093

Jean-Baptiste Voncourt, Frédéric Lionneton, Gueorgui Kratassiouk, François Guillemin, Patrick Netter, Didier Mainard and Jacques Magdalou. (2006) Establishment of a reliable method for direct proteome characterization of human articular cartilage. Molecular Cell Proteomics.

Mannik M, Person RE. (1993) Immunoglobulin G and serum albumin isolated from the articular cartilage of patients with rheumatoid arthritis or osteoarthritis contain covalent heteropolymers with proteoglycans. Rheumatol 13 (3):121-9

Mariella F. Carlevaro, Adriana Albini, Domenico Ribatti, Chiara Gentili, Roberto Benelli, Silvia Cermelli, Ranieri Cancedda, and Fiorella Descalzi Cancedda. (1997) Transferrin Promotes Endothelial Cell Migration and Invasion: Implication in Cartilage Neovascularization. The Journal of Cell Biology 136 (6): 1375–1384

Marta Chevanne, Riccardo Caldini, Giampaolo Manao, Marco Ruggiero. (1999) Heparin binding peptides co-purify with glycosaminoglycans from human plasma. FEBS Letters 463: 121-124

Monika Hermansson, Yasunobu Sawaji, Mark Bolton, Susan Alexander, Andrew Wallace, Shajna Begum, Robin Wait, and Jeremy Saklatvala. (2004) Proteomic Analysis of Articular Cartilage Shows Increased Type IICollagen Synthesis in Osteoarthritis and Expression of Inhibin A(Activin A), a Regulatory Molecule for Chondrocytes. The Journal of Biological Chemistry 279 (42):43514–43521

Muir, H. (1995) The chondrocyte, architect of cartilage. Biomechanics, structure, function and molecular biology of cartilage matrix macromolecules. Bioessays, 17 (12):1039-1048

Saxne T, Glennas A, Kvien TK, Melby K, Heinegard D. (1993) Release of cartilage macromolecules into the synovial fluid in patients with acute and prolonged phases of reactive arthritis. Arthritis Rheum 36 (1):20-25.

Yang Xiang, Taichi Sekine, Hiroshi Nakamura, Shinobu Imajoh-Ohmi, Hiroyuki Fukuda, Kusuki Nishioka, and Tomohiro Kato (2004) Identification of Triosephosphate Isomerase as an Autoantigen in Patients With Osteoarthritis Arthritis & Rheumatism 50 (5): 1511–1521