摘要
原子力顯微鏡在科學上的應用並不只侷限於奈米尺度表面影像的量測，更廣為應用於探索奈米尺度下，生物力學的量測，對研究生物間交互作用有直接的助益。而原子力顯微鏡探針的奈米加工則是利用AFM探針與樣品間的交互作用，改變樣品表面，也同時改變探針尖端的形貌。但由於修飾方法之限制，過去利用原子力顯微鏡研究生物分子多為修飾群體分子於探針上，得到群集反應結果，再藉由統計分析的方式推測單一生物分子的行為表現。
本實驗藉由電場修飾非常微量的金於原子力顯微鏡探針上，再將修飾過的探針經由化學修飾接上蛋白質。由於金的量非常少（假設金球被蒸鍍在探針上為一層單層原子厚度金膜，其面積約為5nm2），利用空間之侷限，極有可能接上大小為10∼15奈米的單一蛋白質。之後，利用全內反射式螢光顯微鏡觀測修飾於蛋白質上的螢光。藉由螢光強度對時間的關係圖，可以得知在全內反射式螢光顯微鏡下的螢光個數。
本研究提出一個修飾探針的新方式，並且可以精準控制修飾於探針的奈米金金數量，並且成功將這個技術延伸至單分子蛋白質檢測。

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