基於數位微流體液滴驅動可應用於正式與非正式科學教育的低成本多功能套件

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研究生：	郭育豪 Guo, Yu-Hao
論文名稱：	基於數位微流體液滴驅動可應用於正式與非正式科學教育的低成本多功能套件 A low cost, versatile kit based on digital microfluidics droplet actuation for formal and informal science education
指導教授：	楊雅棠 Yang, Ya-Tang 黃承彬 Huang, Chen-Bin
口試委員:	何宗易 Ho, Tsung-Yi 莊嘉揚 Juang, Jia-Yang
學位類別：	碩士 Master
系所名稱：	電機資訊學院 - 光電工程研究所 Institute of Photonics Technologies
論文出版年：	2020
畢業學年度：	109
語文別：	中文
論文頁數：	61
中文關鍵詞：	電濕潤、數位微流體、同步微流體驅動、化學教育、晶片實驗室、教育工具
外文關鍵詞：	electrowetting, digital microfluidics, community driven microfluidics, chemical education, lab on a chip, education tool
相關次數：	點閱：3 下載：0
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本文介紹了一種基於數位微流體控制技術的教學套件。並利用化學發光的魯米諾化學式為實例。它還具有螢光成像功能和配備超聲波霧化器的封閉加濕外殼，以防止蒸發。該套件可在短時間內組裝，並且只需要基礎的電子和焊接培訓。該套件使本科和非本科學生，甚至有興趣的人都能以直觀的方式獲得動手操作，並接受培訓以熟悉數位微流體控制技術。

This paper describes an education kit based on digital microfludics. A protocol for luminol-based chemiluminescence experiment is reported as a specific example. It also has fluorescent imaging capability and closed humidified enclosure based on an ultrasonic atomizer to prevent evaporation. The kit can be assembled within a short period of time and with minimal training in electronics and soldering. The kit allows both undergraduate/graduate students and enthusiasts to obtain hands-experience microfluidics in an intuitive way and be trained to gain familiarity with digital microfluidics.

誌謝-----------------------------------II
中文摘要--------------------------------III
Abstract-------------------------------IV
目錄-----------------------------------V
圖目錄---------------------------------VII
表目錄---------------------------------IX
一、緒論--------------------------------1
1-1研究動機-----------------------------1
1-2文獻回顧-----------------------------6
1-2-1微流體實驗製作方法------------------6
1-2-2液滴驅動電路板的建構----------------11
1-2-3 建立簡易且便宜的螢光成像-----------14
二、材料及方法--------------------------15
2-1製作魯米諾實驗樣本--------------------15
2-2製作螢光樣本-------------------------16
2-3微流體控制套件的組裝------------------17
2-4超聲波霧化器的長期液滴驅動實驗---------19
2-5溫溼度環境設計機制--------------------20
2-6系統設計-----------------------------21
2-6-1 電濕潤(EWOD)概念------------------21
2-6-2升壓系統---------------------------25
2-6-3控制電路---------------------------26
2-6-4電路板設計--------------------------27
2-6-5 Arduino程式編譯-------------------30
三、實驗結果-----------------------------32
3-1實驗前準備步驟------------------------32
3-2 魯米諾的化學發光實驗------------------33
3-3 螢光成像實驗-------------------------34
3-4超聲波霧化器的長期液滴驅動實驗結果------35
3-5 套件的穩定性測試----------------------37
四、結論及討論---------------------------39
附錄-------------------------------------40
A-1 Arduino 程式碼-----------------------40
A-2壓克力盒設計圖------------------------46
A-3 NE555P精密計時器的高壓電路------------50
參考文獻---------------------------------56

                                

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