蛇毒金屬蛋白酵素 ( Snake venom metalloproteinases , SVMP : reprolysin ) 於1961年第一次純化出來以後，開啟了此酵素的實驗大門。此酵素與人類之基質金屬蛋白酵素(Matrix metalloproteinases : MMP ) 結構相似，兩者都是需要2價金屬離子 ( Ca 2+ , Zn2+ ) 存在才有酵素活性，其都可能會被EDTA抑制。目前人類至少有28種以上之MMP，可分成 collagenases 、 gelatinases 、 stromelysins 、 membrane type 以及其他類5大類。而蛇類金屬蛋白酵素的分類則依照核苷酸序列長度不同可以分成4類 (N-I~N-IV)，另外以成熟之蛋白大小分類 ， 一樣可以分成4類 (P-I~P-IV)，分類愈後者分子量愈大 ，例如本實驗所要純化的蛇毒金屬蛋白酵素即為P III 型 。
蛇毒金屬蛋白酵素的研究大部分與凝血機制有關，例如所要純化的莫三比克眼鏡蛇蛇毒金屬蛋白酵素，其會切斷血小板上的GPIb，而造成GPIb無法與膜上的VWF factor形成鍵結，而無法進行凝血的步驟。另外此蛇毒酵素被發現能夠切除PSGL-1與骨髓細胞的連結，進而使白血球上的P-selectin無法被辨識，白血球則失去chemotaxis等功用。

本實驗純化的蛇毒金屬蛋白酵素有莫三比克眼鏡蛇，東非眼鏡蛇，及台灣眼鏡蛇。在實驗中利用合成之短鏈□肽當作測試酵素活性的受質，發現三者切PSGL以及GPIb的位置一樣。這些酵素被純化出來後，可以與其他蛇毒成分做實驗，將進一步瞭解蛇毒金屬蛋白酵素的功能。

Abstract
Since the purification of snake venom metalloproteinases（SVMP : reprolysin）, the experimental data have been accumulated. This enzyme is similar to human matrix metalloproteinases (MMP), both need M2+ cation (Ca2+, Zn2+) to be active, this also means that they will be inhibited by EDTA. There are at least 28 kinds of MMP, they can be divided into five groups: collagenases, gelatinases, stromelysins, membrane type and other type. And SVMP can be catalogued into four types according the their nucleotide sequences (N-I ~ N-IV); they can also be grouped into four different types according to the mature protein size (P-I~P-IV), the molecular weight increases with the group number.

The research of SVMP is mostly related the mechanism of coagulation. Take the SVMP of Naja mocambique mocambique as an example, it will cleave GPIb of platelet, making the bonding of GPIb and VWF factor of membrane failed, then the coagulation will be stopped. And SVMP has been found to have other function: it can cleave the linkage between PSGL-1 and myeloid cell, then the P-selectin on the cell surface of leucocyte can not be recognized, and leucocyte can not undergo chemotaxis.

The other kinds of SVMPs to be purified in this thesis are from Naja nigricollis nigricollis and Naja naja atra, but these two SVMP have not been purified yet. Because studies of SVMP of Naja mocambique mocambique are more abundant, hence taking this as the standard for comparison between all these three SVMP, and using synthesized short peptides as the substrates for SVMP to test enzyme activity. After comparing some characteristics between these three SVMPs, we find the active site of these SVMPs might be the same.

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