鐵是維持生命的重要微量元素之一，分析及測定生物體及環境中的鐵物種，對於提升人類健康與生活品質是不可缺乏的重要一環，由於濃度低與易受鹽類基質干擾等因素的限制，發展高效率、低污染風險且操作簡單的樣品前處理系統，對於了解生物醫學與環境科學領域中鐵物種相關研究而言，至今仍是一個十分重要的課題；為解決上述難題，本研究利用PVC粉填充微管柱連結微透析取樣裝置，搭配閥相輔助注入分析系統與感應耦合電漿質譜儀，發展自動化MD-PVC-packed dual minicolumn-ICP-MS連線分析系統，用來監測活體大鼠腦中，因急性去極化效應，所造成腦細胞間液內Fe(II)與Fe(III)的即時動態變化趨勢，根據所獲得之結果說明，當受到急性去極化刺激之後，腦細胞間液內Fe(II)與Fe(III)的濃度確實會發生顯著降低的情形，亦證實此分析系統確實具有監測活體動物腦中鐵物種動態變化趨勢的能力；此外，本研究亦利用三維列印微管柱固相萃取裝置串聯閥相輔助注入分析系統與感應耦合電漿質譜儀，建立自動化3DP-dual minicolumn-ICP-MS連線分析系統，用來分析環境水樣中的鐵物種，根據分析參考樣品（1643e、1640a、SLEW-3與CASS-4）與添加分析試驗之結果，說明本研究所開發之自動化連線分析系統，確實具有準確分析環境水樣中低濃度鐵物種的能力。

Exploration of brain extracellular non-protein-bound/diffusible iron species remains an important issue in investigations of free radical biology and neurodegenerative diseases. In this study, a sample pretreatment scheme, involving poly(vinyl chloride)–packed minicolumn as a selective extraction device, was optimized in conjunction with microdialysis sampling and inductively coupled plasma mass spectrometry (ICP-MS) in cool-plasma mode for in vivo online monitoring of rat brain extracellular Fe(II) and Fe(III). After system’s optimization, the method’s applicability was verified through (i) spike analyses of offline-collected rat brain microdialysates, (ii) determination of the basal Fe(II) and Fe(III) concentrations of living rat brain extracellular fluids, and (iii) monitoring of the dynamic changes in the Fe(II) and Fe(III) concentrations in response to perfusion of a high-K+ medium.

Furthermore, to enable speciation of trace iron in environmental samples, a stereolithographic 3D printer and acrylate-based resins were utilized to fabricate a demountable minicolunm to selectively extract Fe(II) and Fe(III) and facilitate their analyses in high-salt-content environmental samples by hyphenating to ICP-MS. After system’s optimization, the analyses of the reference materials 1643e, 1640a, CASS-4, and SLEW-3 as well as the spike analyses of those collected environmental samples were performed to confirm the method’s reliability. These two proposed sample pretreatment schemes, based on respectively using the PVC-packed minicolumn and the non-functionalization 3D-printed minicolumn, appear to have great practicality for the iron speciation in biological and environmental samples.

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