呼吸作用作為細胞代謝產生能量的過程，有許多針對其代謝途徑及產物的研究。 本文介紹一系統透過生物反應器進行培養及排放的二氧化碳濃度偵測，以及將 OD600 換算出乾燥重量進行更進一步的分析。利用半導體製程中常用的真空系統進行不同碳源之大腸桿菌培養實驗，其能夠提供較佳的氣密性以供生物反應器進行氣體濃度量測， 並結合光密度測量 OD600、流體攪拌、二氧化碳氣體量測以及資料無線傳輸等功能。

As respiration being a process of cellular metabolism for energy production, many studies have focused on its metabolic pathways and products. In this research, we first introduce a system performing bacteria culture and emitting CO2 detection using our bioreactor, then OD600 is converted to dry weight for further analysis. Escherichia coli was cultured with different carbon sources utilizing the vacuum system which is commonly used in semiconductor manufacturing processes .The vacuum system provides a better air tightness for our bioreactor to detect gas cancentration, which combined optical density(OD600) detection, fluid mixing, CO2 concentration detection and wireless transmission.

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