中藥三七(panax notoginseng)除了傳統上用於止血及改善心血管之功能外，在近年來被發現具有抗氧化及保護神經細胞之潛力，此次的研究中，我們使用酒精萃取的方式提取含有三七皂苷之三七乙醇提取物(PNE)，並利用釀酒酵母(saccharomyces cerevisiae)作為模式生物檢視三七乙醇萃取物之抗氧化能力，在外源性的氧化刺激方面，預先加入三七乙醇萃取物能夠協助酵母菌提升其在暴露致死劑量過氧化氫及熱休克後之存活率，透過染色及流式細胞儀分析，我們發現三七乙醇能降低過氧化氫及熱休克所誘導的活性氧化物質積聚，在迫使酵母菌進行呼吸作用的條件下，三七乙醇萃取物也能夠減少酵母菌細胞因呼吸作用產生之活性氧化物質在細胞內積聚之情況，最後利用老化酵母細胞篩選的技術，我們將細胞族群中老化的酵母細胞進行分離並且分析，我們發現三七乙醇萃取物能降低老化酵母細胞活性氧化物質的積聚，透過此次的研究，我們發現在酵母菌模型中，三七乙醇提取物能提升酵母菌對於熱休克及過氧化氫所帶來氧化壓力之抗性，並降低呼吸作用以及衰老所產生的活性氧化物質在細胞內積聚的狀況。

In addition to the traditional use of hemostasis and improve cardiovascular function. Panax notoginseng in recent years have been found to have the potent of antioxidant and neuron protection. In this study, we used alcohol extraction to obtain the ethanol extract (PNE) containing Panax notoginseng Saponins (PNS) and used the Saccharomyces cerevisiae to examine the antioxidant capacity of the PNE. Pretreatment with PNE can assist the yeast cells to enhance its survival rate at exposure to lethal dose of oxidative stress. By using flow cytometry, we found that PNE can reduce the accumulation of reactive oxygen species induced by hydrogen peroxide and heat shock. PNE can also reduce the accumulation of ROS produced by respiration in the yeast cells. Finally, using the technique of sorting the aged yeast cells, aging yeast cells were isolated and analyzed. We found that PNE can reduce the accumulation of ROS in aged yeast cells. In this study, we suggested that in the yeast model, the PNE could increased the ability of yeast to resist the oxidative stress caused by heat shock and hydrogen peroxide, and reduce the accumulation of ROS cause by respiration and senescence.

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