組織工程是近年來新興的生醫技術，為結合臨床醫學應用與生醫材料知識，利用細胞、人工細胞外間質與生長信息等要素，在體外培養出人體組織或器官的科學。然而在植入生物體內後，這些以體外再生方式培養出來的組織往往會因為短期內宿主的血管新生現象不足，無法獲得充分的氧氣與養分而壞死。為了改善植入組織內部的血管新生情形，本論文研發出一項新的組織工程技術，以本實驗室先前開發的多孔性去細胞牛心包膜做為組織工程支架，並結合含有臍帶血間葉幹細胞(cord-blood mesenchymal stem cell, cbMSC)與人類臍帶靜脈內皮細胞(human umbilical vein endothelial cell, HUVEC)的細胞片，使支架能夠預血管化(prevascularization)，加快移植後其內部的組織再生與血管新生。第一部份的實驗裡，我們利用體外培養的方式，評估cbMSC對HUVEC在管狀形成實驗(tube formation assay)中的影響，發現加入適量的cbMSC可有效延長管狀結構存在的時間。另一方面，我們以細胞片培養系統建立HUVEC/cbMSC細胞片，觀察細胞貼附及生長情形，並以免疫染色鑑定其細胞外間質的分佈，可知由降溫脫附而得到的細胞片可保存大量的細胞外間質。而經過數天的共培養後，cbMSC的α-smooth muscle actin表現量顯著上升，表示有部份的cbMSC可能開始朝平滑肌細胞分化。在體內實驗部份，我們將HUVEC/cbMSC細胞片夾附至多孔性去細胞牛心包膜支架後，植入老鼠背部的皮下組織，以探討該細胞片在生物體內對於血管新生與組織再生的影響。實驗結果顯示，HUVEC/cbMSC細胞片可以促進支架內部的血管新生，並誘導宿主細胞遷入，第十四天時大部分的孔洞皆已被細胞填滿；而未夾附細胞片的支架內部仍有許多空隙。上述體外與體內實驗的結果顯示，將牛心包膜支架夾入HUVEC/cbMSC細胞片，可有效加快移植後支架內部的血管新生，達到組織修復與再生的目的。

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