一般傳統蛋白質免疫分析法，應用血清作鍵結阻絕(Blocking)，利用液體將表面反應官能基( surface reactive fuctional group)阻絕，而本研究專注於運用氣相蒸鍍小分子，使小分子得以與表面官能基反應，達到鍵結阻絕效果。利用傳統方式作鍵結阻絕，容易造成拖尾及交互汙染等問題，而且一般血清為大分子，以小牛血清(Bovine Serum Albumin, BSA)為例，其分子重量(molecular weight)約為66KD，會造成鍵結阻絕過程中，阻擋後續抗體進行辨認的因素。而利用蒸鍍小分子，不僅可減少交叉汙染的影響，且在適量的時間調控下，可以避免拖尾的現象。本研究使用的小分子，分子量為481.54遠比傳統血清小，在適當時間操作下，可避免部分由生物血清造成辨認障礙的問題。
本研究運用蒸鍍小分子達到鍵結阻絕的改善，比較純化檢體與血清檢體的檢測效果，在血清檢體下，氣相鍵結阻絕試劑可以提高噪訊比。因此，提出了氣相阻絕試劑，在血清系統中，由於背景值降低，訊號值升高，因此可提高噪訊比，提高檢測靈敏度。且本研究發現，傳統BSA阻絕在血清系統下，訊號值較純化系統提高很多，代表沾黏狀況嚴重，而氣相阻絕試劑在血清系統下訊號值相對來說增加比較少，因此提出氣相阻絕試劑比傳統生物血清阻絕效果好。

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