熊果素為植物中萃取的一種美白成分。由於萃取的產量極低，故以醣化進行結構修飾將對苯二酚轉換為熊果素為較經濟可行之方法。Kurosu等人於2002年成功的利用微生物Xanthomonas campestris WU-9701做為生物觸媒，並以麥芽糖作為醣化反應之醣基來源進行對苯二酚之醣化反應，生產熊果素達轉化率達93%。然而在具醣化催活性之菌種篩選上，至今仍尚無簡單快速之篩選方法。因此，本研究目的在於建立一簡單、高效率之篩菌策略，以期經由突變及菌種篩選獲得具有醣化對苯二酚催化活性之高活性菌株。本實驗所建立的篩菌策略是利用對苯二酚相較於熊果素其光敏感性較高之特性（對苯二酚溶液照光後較容易變質而使溶液呈現深褐色），於96孔盤反應後進行光照，以顏色深淺作為指標進行初篩。本實驗經過五次的突變、多次的菌種篩選以及反應條件之最適化探討後，已獲得轉化率約85%的高活性菌株Xanthomonas campestris 5H10。

Arbutin is a whitening agent, which is extracted from plants and the yield is quite low. A more economical and environmental friendly way is to synthesize arbutin by structural modification of hydroquinone via glycosylation(biotransformation). Presently, Kurosu and coworkers(2002) have successfully synthesized arbutin by glycosylation of hydroquinone with maltose as a glucosyl donor using lyophilized cells of Xanthomonas campestris WU- 9701 as the biocatalyst. However, a fast and simple screening method for microorganisms which have catalytic activity of glycosylation has not been established up till now. So, the objective of our study was to set up a easy and efficient screening method for obtaining mutated strains that have high hydroquinone-glycosylation activity. Our screening method was based on the fact that hydroquinone solution was more light-sensitive than arbutin. We selected the mutated strains from 96-well plates after reaction and light exposure and used the color difference as the screening index. After five rounds of mutation, repeated screening and optimization of reaction conditions, we obtained a high activity strain, Xanthomonas campestris 5H10, which has a conversion of about 85%.

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