研究生: |
王欽世 Wang, Chin-Shyh |
---|---|
論文名稱: |
以幾丁聚醣及明膠作為高分子/陶瓷複合式支架軟骨層的研究 A study of chitosan and gelatin as the cartilage layer of polymer/ceramic composite scaffold |
指導教授: |
黃大仁
Huang, Da-Jen |
口試委員: | |
學位類別: |
碩士 Master |
系所名稱: |
工學院 - 化學工程學系 Department of Chemical Engineering |
論文出版年: | 2009 |
畢業學年度: | 97 |
語文別: | 中文 |
論文頁數: | 118 |
中文關鍵詞: | 軟骨 、明膠 、幾丁聚醣 、貼附率 、連結力 |
外文關鍵詞: | Cartilage, Gelatin, Chitosan, Rate of cell-attached, Link force |
相關次數: | 點閱:2 下載:0 |
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本實驗室研究重心為軟骨細胞支架,改質明膠支架,嘗試將明膠支架與天然交聯劑Genipin(GP)進行交聯作用,得到低生物毒性、多孔性、及高內部連通性的明膠支架,且改善了未交聯之明膠支架其機械性質差及降解速率快等問題。而後,將明膠支架連結於tricalcium phosphate (TCP)及calcium polyphosphate (CPP)層上,製作出硬骨層-分隔層-軟骨層等三層的複合型陶瓷支架。不過,明膠與TCP之間的連結力並不高,找出一個替代的生醫材料或連結方式是一個探討方向。
本論文將幾丁聚醣與明膠作為陶瓷支架軟骨層,培養軟骨細胞,發現幾丁聚醣含量多的支架細胞貼附不易,細胞型態不佳,但glycosaminoglycan (GAG)分泌較良好;明膠含量多的支架細胞貼附率高,細胞生長良好,唯獨GAG分泌量較低。利用fibronectin改質幾丁聚醣支架,雖然可以提升細胞貼附率,但對於細胞的生長沒有明顯幫助。
連結力方面,在直接接著情況下,TCP與幾丁聚醣連結力較明膠與明膠來得好,但利用PLGA接著軟骨層與分隔層可以明顯提升兩者的連結力。
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