軸突上的局部蛋白質合成對於軸突生長、方向引導與突觸建立都扮演著重要的角色，但是軸突上是否具有可以修飾和運送蛋白的高爾基體一直受到長時間的質疑。雖然早期的微結構研究表示軸突沒有高爾基體，但是透過蛋白質體學和生化的分析，在軸突上發現許多和高爾基體相關的蛋白，這樣的證據間接顯示了軸突可能具有高爾基體或是具有類似於細胞本體觀察到的高爾基體結構。在本篇研究中，我們在玻璃圓玻片上培養大鼠皮質神經元，並利用poly-L-lysine壓印出本實驗室設計的微接觸壓印圖案，使用經過處理後的玻片使我們可以更容易觀察到軸突和軸突生長錐。利用免疫螢光染色的方式偵測軸突生長錐上Golgi marker的螢光反應，透過觀察染色結果發現神經細胞與軸突生長錐上的高爾基體的染色型態差異。在共定位分析的實驗中，我們發現生長錐上的高爾基體標記物和內質網之間並沒有顯著的共定位，另外也比較近端和遠端高爾基體標記物的分佈情形，得到的結果是一致的。此外，我們無法確定高爾基體蛋白的染色是否和微管或F-肌動蛋白有關。本研究得到的結果初步確認了軸突生長錐上高爾基體蛋白的存在，並且高爾基體蛋白的分布不同於細胞本體中的結果。未來可以進一步去探討軸突高爾基體的功能，也有助於在電子顯微鏡中觀察軸突高爾基體的結構。

Local protein synthesis in the axon participates in axonal growth and guidance and synaptic connection establishment. Whether the Golgi apparatus exist in the axon for local protein maturation has been an unanswered questioned thus far. While ultrastructural studies suggest that axons do not have Golgi apparatus, many Golgi-related proteins were found on axons by proteomic and biochemical analysis of axon. These results suggests that axons may have a Golgi apparatus, or a structure equivalent to the Golgi apparapus as that observed in the cell body. In this study, I grew rat cortical neurons on glass coverslips with a poly-L-lysine-coated micropattern use made by microcontact printing on the surface. The use of these glass coverslips allows me to observe axons and axonal growth cones easily. Immunofluorescence staining axonal growth cones using antibodies to well-characterized Golgi markers was conducted here. I observed different immunostaining patterns of these Golgi markers in the cell body and axonal growth cones. By colocalization analyses, I found that there is no significant colocalization between the immunoreactivity of the markers of Golgi apparatus and the endoplasmic reticulum in the growth cone. Furthermore, I could not ascertain whether the immunostaining of Golgi protein is associated with microtubules or F-actin. My results that the axonal growth cones contain Golgi proteins and that the distribution of Golgi proteins is unlike that of their distribution in the cell body existence of the Golgi apparatus-like structure in the axonal growth cone. In the future, it will be of interest to study the structure of the Golgi apparatus-like structure in the axon by electron microscopye.

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