拉曼散射 (Raman scattering) 顯微術是一項生物成像技術。不同於螢光顯微鏡需倚賴螢光標記標定檢測目標，拉曼散射的訊號是來自於樣本本身的化學鍵，因此可以在無標記 (Label-free) 的情況下取得具有高特徵性的影像。
　　拉曼散射顯微術當中的同調拉曼散射 (Coherent Raman scattering, CRS) 顯微術，可以在無標記的情況下獲得活體細胞的實時 (Real time) 影像。由於同調拉曼散射需要波長能夠調變的光源，我們首次利用多重薄片展頻 (Multiple-plate continuum, MPC) 技術所產生的超寬頻 (supercontinuum) 光源，搭建一套可以同時掃同調反斯托克斯散射 (Coherent anti-Stokes Raman scattering, CARS) 顯微術以及受激拉曼散射 (stimulated Raman scattering, SRS) 顯微術這兩種同調拉曼散射的影像系統。我們比較兩種技術的差別，並擷取兩種無標記果蠅腦的同調拉曼散射影像。

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　　Raman scattering microscopy is a biological imaging technique. Unlike fluorescence microscopy, which relies on fluorescent probes to label detection targets, Raman scattering signals stem from chemical bonds in the sample itself. Thus, it can obtain high-characteristic images without labeling (Label-free).
　　Coherent Raman scattering (CRS) in Raman scattering microscopy can conduct real-time live-cell imaging. Since coherent Raman scattering requires a tunable wavelength, we utilize a supercontinuum light source generated by a multiple-plates continuum (MPC) technique to provide arbitrarily tunable wavelengths. Also, we used this light source to build a system that can measure two coherent Raman images simultaneously that are Coherent anti-Stokes Raman scattering (CARS) microscope and Stimulated Raman scattering (SRS) microscope, respectively. We compared the differences between the two techniques and collect coherent Raman scattering images of Drosophila brains

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