本研究使用自由基ABTS•+ (2,2’-azinobis-(3-ethylbenzothiazoline-6- sulfonate))後衍生分析系統搭配毛細管區帶電泳(Capillary Zone Electrophoresis；CZE)分析具自由基抓取能力之抗氧化物。實驗主要分成兩大部份：前者為量測抗氧化能力之微量流洗系統建立，後者為毛細管電泳搭配此微量流洗後衍生系統之研究。微量ABTS•+流洗系統在線上偵測樣品抗氧化能力時顯示了高再現性與高靈敏度，偵測極限(S/N=3)約10 μM (進樣量100 nL)，且1小時內可連續偵測40個樣品，適合用來處理大量飲食樣品之監測。在毛細管電泳後衍生系統中探討了不同流洗緩衝液濃度、pH值、電壓等變因，在最佳化條件下ABTS•+試劑成功地與毛細管電泳結合，簡單有效地分離5種混合樣品 (benzoic acid, sorbic acid, L-(+)-ascorbic acid, D-(-)-isoascorbic acid, gallic acid)並能同時偵測其抗氧化能力，電泳分離時間不超過17分鐘，其抗氧化能力分析的偵測極限(S/N=3)約8 μM (進樣量50nL，使用414 nm為吸收峰)；測量值與文獻使用傳統UV測量方法相符合。整體而
言，此系統能夠有效分析測量樣品之抗氧化能力並應用於實際物種之量測。

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