根據台灣衛生福利部於2018年的統計中指出，惡性腫瘤在台灣十大死因中位居第一，子宮頸癌在台灣女性癌症排名中則位居第七。人類乳突病毒 (HPV)的感染是造成子宮頸癌的主因，初期症狀不容易被診斷出來，一旦子宮頸癌發生轉移的現象該患者之五年存活率會降低許多。而罹患子宮頸癌的女性中神經內分泌癌之發生率則位居第三，屬於罕見的子宮頸癌類型，與鱗狀細胞癌和腺癌相比是一種較為惡性的腫瘤型態。因此，為了更有效治療罕見發生的神經內分泌子宮頸癌，了解神經內分泌子宮頸癌與其他類型子宮頸癌不同的分子作用機制是重要的任務。在先前的研究中利用二維差異電泳 (2D-DIGE)與基質輔助雷射脫附游離飛行時間質譜儀 (MALDI-TOF-MS)分析神經內分泌子宮頸癌細胞 (HM-1)與其他三種子宮頸癌細胞 (Caski、ME-180、HeLa)之間蛋白質表現的差異，挑選出在HM-1細胞中具有高度蛋白表現量的Transgelin及Galectin-1作為後續研究的標的。在本研究中，使用從人類組織中分離的神經內分泌子宮頸癌細胞HM-1及HM-2，利用RNA干擾技術抑制Transgelin及Galectin-1蛋白表現量，Transgelin基因被沉默後能夠有效抑制神經內分泌子宮頸癌細胞的侵襲能力，也會導致細胞週期的停滯、促進細胞凋亡進而導致細胞增殖能力的下降，影響TGB-β/Smad路徑的訊號傳遞；Galectin-1基因被沉默後也能夠抑制神經內分泌子宮頸癌細胞的爬行能力及細胞增殖能力降低。透過本研究的結果可以知道Transgelin及Galectin-1都會影響神經內分泌子宮頸癌的爬行能力及增殖能力，針對神經內分泌子宮頸癌的檢測或是治療都是很好的蛋白標的。

According to the statistics of Ministry of Health and Welfare in 2017, malignant tumor is ranked first in ten leading causes of the death. Cervical cancer is ranked seventh in the ranking of female cancer. Infection of HPV is the main cause of cervical cancer. In the early stage, it is hard to be diagnosed. Moreover, the metastasis of cervical cancer decreases five-year-survival rates. Neuroendocrine cervical cancer is ranking on the third position. It belongs to rare type of cervical cancer and also be the more malignant cancer type, compared to squamous cell carcinoma and adenocarcinoma. In order to have a effective treatment in neuroendocrine cervical cancer, molecular mechanism is necessary to be researched. In the previous study, it performs 2D-DIGE and MALDI-TOF-MS to analyze the differentially expressed proteins in neuroendocrine cells (HM-1) and other types of cervical cancer cells (Caski, ME-180, HeLa). Transgelin and Galectin-1 are selected as the candidate proteins because of high protein expression in HM-1 cells. In this study, use small interfering RNA (siRNA) to knockdown Transgelin and Galectin-1 expression in HM-1and HM-2 which isolated from human tissue. The migration ability and proliferation ability of neuroendocrine cervical cancer cells are effectively inhibited. Also, signal transduction of TGB-β/Smad pathway is inhibited. After Galectin-1 being knockdown, it inhibits migration and proliferation in neuroendocrine cervical cancer cells. As a result, Transgelin and Galectin-1 affect the migration and proliferation ability in neuroendocrine cervical cancer. There are protein targets in detection and treatment of neuroendocrine cervical cancer.

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