本研究是以戊二醛交聯聚乙烯醇(PVA)與硫酸軟骨素(CS)的方式製備水膠，預期所得水膠將兼具兩者之優點，可用作組織工程基材。
經過紅外線光譜、核磁共振光譜及基材切片的鑑定，PVA-CS水膠應是成功地被製備。從物理性質來看，PVA-CS水膠的澎潤度會隨著CS含量上升；拉力係數則隨著CS含量下降；熱穩定性受到CS的影響略微下降。
相較於高親水性不易吸引細胞貼附的PVA水膠，PVA-CS水膠不但有利於細胞貼附，還可促進細胞生長，以表現最好的CS20水膠來說，貼附在基材表面的BHK細胞彼此連接增生成緻密細胞層，其細胞數目是PVA水膠的2.56倍，從細胞測試顯示CS能有效地促進細胞在基材表面生長。
軟骨素能增進細胞與基材間互動，達到促進細胞生長的目的，而PVA本身的強度也提供了基材足以應付體外細胞培養所需的機械性質，由上述測試可知PVA-CS水膠有應用在組織工程的潛力。

The PVA-CS hydrogels in the research is prepared by use of glutaraldehyde, the cross-linking agent. The obtained hydrogels, holding both advantages of PVA and CS, are expected to be the scaffolds of tissue engineering.
The PVA-CS hydrogels should be successfully prepared according to the evidence of IR, Solid NMR and the slide of scaffold. In terms of physical properties, the swelling ratio increased as the amount of CS; the addition of CS weakened the scaffold and led to the smaller stretching modulus; the heat stability of PVA-CS slightly dropped as compared with PVA.
The PVA-CS hydrogels not only promote cell adsorption, but also cell growth. The cells adhered to the CS20 hydrogel connected together and formed a dense sheet of cells. The cell number of CS20 hydrogel was 2.56 times PVA hydrogel, hydrophilic and unfavorable for cell adhesion. Cell test shows that CS involved in the scaffolds effectively promote the growth of BHK cells on the surface.
CS really enhances the interaction between cells and scaffold and reaches the goal of promoting cell growth. Further, the strength of PVA itself also affords the scaffolds enough mechanical properties for cell culture in vitro. As a result of tests above, the PVA-CS hydrogels have the potential for tissue engineering.

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