在自然界及農業上植物常常遭受到低溫的逆境，導致不論在植物本身以及農業利益上造成重大傷害。因此瞭解植物如何遭受寒害，以及低溫下植物如何適應和馴化機制則相當重要。我們利用PCR-select cDNA subtraction方法從低溫敏感的綠豆品種Vigna radiata VC1973A cultivar篩選出263個可能受低溫逆境誘發的基因片段（EST）。結合實驗室所有綠豆的ESTs，一共1,022 ESTs並建立cDNA微陣列晶片，以利在逆境下同時觀察大量基因表現情形。
接著為了瞭解多元胺以及乙烯在低溫下所扮演的角色，及兩者代謝上的相互關係，甚至瞭解低溫馴化過程中低溫敏感（VC1973A）及低溫不敏感（NM94）綠豆品種間之多元胺角色的差異。我們選擇乙烯及多元胺代謝路徑上三個重要的基因，VrSAMDC (S-adenosylmethionine decarboxylase), VrSAMS (S-adenosylmethionine synthetase), 以及VrACO (1-aminocyclopropane-1-carboxylic acid oxidase) ，利用北方墨點法及逆轉錄PCR方法觀察在低溫下不同時間點其轉錄層次基因表現情形，並探討低溫敏感綠豆品種VC1973A和低溫不敏感品種 NM94 cultivar兩者間的基因表現異同。

Plants are frequently exposed to low temperature stress both in natural and agricultural conditions, which often result in great damage to plant yield and to people demand. Thus, understanding the physiological processes that underline stress injury and adaptation and acclimation mechanisms of plants to environment stress is of immense importance to both agriculture and the environment. PCR-select cDNA subtraction was performed to isolate the cold inducible genes in mungbean (Vigna radiata) VC1973A cultivar (chilling-sensitive). A total of 263 ESTs was obtained and combined to other mungbean ESTs in our laboratory. To sum a total of 1,022 ESTs and all subjected to cDNA Microarray analysis. In order to understand the role of polyamines and ethylene between cold-sensitive and cold-tolerant plant during cold acclimation, we applied Northern blot and RT-PCR to investigate the temporal pattern of VrSAMDC (S-adenosylmethionine decarboxylase), VrSAMS (S-adenosylmethionine synthetase), and VrACO (1-aminocyclopropane-1-carboxylic acid oxidase) transcripts during exposure to low temperature both in VC1973A cultivar (cold-sensitive) and NM94 cultivar (cold-tolerant).

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