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研究生: 陳映竹
Chen Ying-Chu
論文名稱: 以介電泳方式操控在微流道環境下生成包裹細胞之微液珠
Manipulation Cell-Containing Droplets using Dielectrophoresis(DEP)
指導教授: 陳致真
口試委員: 陳景欣
鄭兆珉
學位類別: 碩士
Master
系所名稱: 工學院 - 奈米工程與微系統研究所
Institute of NanoEngineering and MicroSystems
論文出版年: 2013
畢業學年度: 101
語文別: 中文
論文頁數: 94
中文關鍵詞: 微流道液珠
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  • 微液珠技術可將細胞或生化物質包裹於奈升至皮升的微液珠中,並可以進行操控及觀察。此技術之應用包括以細胞為模型之藥物檢測、毒性檢測,各種生化物質之反應以及可用來做細胞培養。
      生成微液珠的微流道孔徑為微米等級,因此所需之藥劑量與試劑量相對於傳統技術減少許多,同樣容量利用微液珠可增加至少十萬倍(從微升至奈升及皮升)的檢體及實驗。
      於目前研究分離包裹細胞微液珠的文獻中,大部分是以類似迴授的方式,先檢測在選擇所要分離的微液珠,或者直接將生成出的微液珠分成兩類,篩選去除不需要的微液珠,簡而言之,不論是哪一種方式,每次都只能取得一種形式的微液珠。
      因此本論文提供一種新的想法,以介電泳力的方式,液珠中包裹細胞的數目不同,因此極化的程度不同時所需之介電泳力也不相同,希望利用此不同,能達到將相同包裹細胞數目的微液珠分離到同一個子流道,利用介電泳力之方式,每個包裹不同數量細胞微液珠就會往不同的方向分離至不同的子流道。
      在本論文,我們成功的移動有裹細胞之液珠,並在未來會將電極之設計優化,將空液珠與有包裹細胞之液珠之差別突顯出來,希望能成功將液珠分離至不同的子流道。


    第一章 前言 1 1.1前言及研究背景 1 1.2 研究動機 2 第二章 文獻回顧 4 2.1 液珠之形成 6 2.1.1 聚焦型流道 6 2.1.2 T型流道 8 2.2 界面活性劑 10 2.3 包裹細胞的液珠 11 2.4 分離液珠 12 2.4.1 幾何形狀方式 12 2.4.2 光學方式 13 2.4.3 磁力方式 15 2.4.4 介電泳方式(Dielectrophoresis,DEP) 16 2.4.5 其它方式 18 第三章 相關介電泳基本原理 19 3.1 電泳 19 3.2 介電泳理論 19 3.3 介電泳力數學模型 22 第四章 晶片設計與製作 25 4.1晶片設計概念 25 4.1.1 電極之設計 26 4.2 光罩製作 28 4.3 晶片製作 31 4.3.1 黃光微影製程 31 4.3.2 PDMS翻模 33 4.3.3 ITO玻璃電極蝕刻 35 4.3.4 氧電漿結合 36 第五章 實驗結果 37 5.1微流道晶片製作結果 38 5.2液珠生成 44 5.3流速比對液珠生成之影響 48 5.4 介面活性劑對液珠生成的影響 50 5.5 液珠之包裹 56 5.5.1 細胞之培養 59 5.6 介電泳分離液珠之想法 61 5.6.1 流道電場模擬 65 5.6.2 操縱液珠之實驗 71 5.7 通過微流道之細胞培養 79 第六章 結論與討論 86 6.1 總結 86 6.2 問題的解決與分析 86 6.3 討論與改進 88 參考資料 91

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