氫氧基磷灰石 (Hydroxyapatite, HAp)為人體骨骼中主要的無機成分，具有良好的生物相容性，臨床上常被應用在骨科及牙科方面。本研究以溶膠-凝膠法合成具有抗菌性之氫氧基磷灰石(HAp)粉體與覆層，並且進行覆層與齵齒病原菌Streptococcus mutans的抗菌實驗，及細胞毒性測試。
在不同銀含量的HAp覆層中，添加100 ppm銀離子於前驅物中所得的覆層，即可產生抑菌效果，10000ppm銀離子的添加可在固態培養基上產生明顯的抑菌環。在不同鋅含量的HAp覆層中，只有添加10000 ppm鋅離子的覆層才能產生抑菌效果。此外，在製備完成的HAp塗層表面上塗佈抗生素(PSA；Penicillin，Streptomycin Amphotericin)也可產生抑菌環。初步的細胞毒性測試結果顯示，比較添加相同濃度之銀、鋅於前驅物的覆層時，添加鋅者較利於人類齒齦纖維母細胞(HGF-1)之貼覆生長。

所以在考量抗菌效果、生物相容性及低成本的牙科植體材料上，未來可製備混合添加微量銀及少量鋅元素之覆層，鋅用量雖需高於1%，但便宜、無毒且可促進HGF-1生長，未來可嘗試100ppm Ag +10000ppm Zn之配方。

Hydroxyapatite (Ca10(PO)6(OH)2, HAp) is known for its biocompatibility in calcified tissues, and has been prepared in many forms for orthopedic and dental applications. HA is always directly implanted into the body, so that HAp with anti-bacterial property will extend its application and benefit the storage.
In this study, our aim is to prepare effective antibacterial HAp powders and HAp coating on Ti-6Al-4V. Sol-gel method was employed to synthesize Ag-doped or Zn-doped HAp films, and the antibacterial test of HAp versus S. mutans was performed. With an addition of 100 ppm Ag+ ions in the sol-gel precursor, calcined HAp films showed growth suppression of the pathogen. Apparent inhibition zone was found for HAp film with increased Ag+ ions addition of 10000 ppm. But for the addition of Zn2+ ions, the inhibition zone can be found only when adding 10000 ppm and more Zn2+ ions to HAp precursor. The cytotoxicity tests showed that the HAp films with Zn2+ ions addition are better for the attachment and spread of human gingival fibroblasts (HGF-1).

In order to reduce production cost of dental implants for future applications, addition of both Ag and Zn in to HAp films is suggested.

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