本研究利用新型光生化反應器培養藻類生產油脂，並將其作為原料轉化為生化柴油。此反應器主要以直立網板為中心，藻液在網板上形成薄膜流動，如此可以改善一般傳統開放式反應器光線分部不均勻、攪拌不完全和單位土地面積的生產量過低的缺點。本研究由七隻微藻篩選出油脂含量高達63﹪的Nannochloropsis oculata，探討其最適化的培養環境。最後將Nannochloropsis oculata培養於直立網板反應器上，探討直立網板反應器的操作條件，並與平面培養池比較，其最終生質量約為平面培養池的兩倍，證明直立網板反應器確實提升單位土地面績的利用效率。

Reference
Amos Richmond, 2004, handbook of microalgal culture biotechnology and applied phycology, 1st edition, Blackwell Science
Baum R., 1994, Microalgae are possible source of biodiesel fuel. Chem. eng. News.72,28-29
Becker, E. W., 1994, Microalgae : biotechnology and microbiology, Cambridge University Press, pp.1
Ben-Amotz, A., Tornabene, T. G., 1985. Cemical profile of selected species of microalgae with emphasis on lipid. J. Phycol., 21, 72-81.
Binaghi L., A. Del Borghi, A. Lodi, A. Converti *, M. Del Borghi, 2003, Batch and fed-batch uptake of carbon dioxide by Spirulina platensis. Process Biochem. 38,1341-1346
Camacho Rubio F., F. G. Acie´n Ferna´ ndez,J. A. Sa´ nchez Pe´ rez,F. Garcia Camacho,E. Molina Grima2,1999, Prediction of Dissolved Oxygen and Carbon Dioxide Concentration Profiles in Tubular Photobioreactors for Microalgal Culture. Biotechnol Bioeng 62,71–86.
Connemann J., J. Fischer, Biodiesel quality Y2K and market experiences with FAME, CEN/TC 19 Automotive Fuel Millennium Symposion, Amsterdam, The Netherlands, 1999, 25-26.
Denich T.J., L.A. Beaudette, H.Lee, J.T.Trevors, 2003, Effect of selected environmental and physico-chemical factors on bacterial cytoplasmic membranes, J. microbiol. methods.52, 149-182
Kaia Roman, 2000, From the Fryer to the Fuel Tank The complete guide to using vegetable oil as an alternative fuel, 3rd edition, Joshua Tickell
Kenneth L. Terry and Lawrence P. Raymond, 1985 system design for the autotrophic production of microalgae. Enzyme Microb. Technol., 7, 474-487
Kimura K. M. Yamaoka, Kamisaka Y., 2004 Rapid estimation of lipids in oleaginous fungi and yeasts usingNile red fluorescence Journal of Microbiological Methods 56, 331– 338
Masahiko Moitia, Toshitomo Watanabe, Hiroshi Saiki, 2000, Investigation of photobioreactor design for enhancing the photosynthesis productivity of microalgae. Biotechnol. bioeng. 69, 693-698
Martin-Jezequel V, Hildebrand M, Brzezinski MA, 2000, Silicon metabolism in diatoms: Implications for growth. J. phycol. 36, 821-840
Mazzuca T. Sobczuk, F. Garcı´a Camacho, F.Camacho Rubio, F. G. Acie´n Ferna´ ndez, E. Molina Grima, 1999, Carbon Dioxide Uptake Efficiency byOutdoor Microalgal Cultures in TubularAirlift Photobioreactors. Biotechnol. bioeng.67, 465-475
Piorreck, M., Baasch, K. H., Pohl, P., 1984. Biomass production, total protein, chlorophylls, lipids and fatty acids of freshwater green and bule-green algae under different nitrogen regimes. Phytochemistry 23, 207-216
Pulz O.,2001, Photobioreactors: production systems for phototrophic microorganisms. Appl. Microbiol. Biotechnol. 57, 287-293
Radmer, R. J., Parker, B. C., 1994. Commercial applications of algae - opportunities and constraints. J. Appl. Phycol. 6, 93-98.
Rados, S., Vaclav, B., Frantisek, D., 1975. CO2 balance in industrial cultivation of algae. Arch. Hydrobiol. 46, 297-310.
Renaud SM, Parry DL,1994, Microalage for use in tropical aquaculture 2.effect of salinity on growth, gross chemical-composition and fatty-acid composition of 3 species of marine microalgae. J. appl. Phycol. 6, 347-356
Renaud S.M., Zhou H. C., Parry D. L., Lunong-Van Thinh & Woo K. C., 1995, Effect of temperature on the growth,total lipid content and fatty acid composition of recently isolated tropical microalge Isochrysis sp., Nitzschia closterium, Nitzschia paleacea, and commercial species Isochrysis sp. J. appl. phycol.
Richard J. Geider and Bruce A. Osborne, 1992, Algal Photosynthesis, Chapman and hall
Schpeper T., Al-Rubeai M., Corne J. F. t, Dussap C. G., Elias C. B., Gomes J., J.-B. Gros, Hill D. C., Joshi J.B., Menawat A.S., Nisbet L. J., Pulz O., Scheibenbogen K., Wrigley S. R., 1998, Bioprocess and algae Reactor Technology, Apoptosis, Springer
Shifrin, N. S., Chisholm, S. W., 1981. Phytoplankton lipids : interspecific differences and effects of nitrate, silicate, and light-dark cycles. J. Phycol. 17, 374-384
Sriharan, S., Bagga, D., Sriharan, T.P., 1989, Environmental control of lipids and production in the diatom Navicula saprophila. Appl. Biochem. Biotechnol. 20/21, 281-291.
Sukenik, A., Carmeli, Y. & Berner, T., 1989, Regulation of fatty acid composition by irradiance level in the eustigmatophyte Nannochloropsis sp. J. Phycol., 25, 686-692
Susan M. Renaud*, Luong-Van Thinh, George Lambrinidis, David L. Parry, 2002, Effect of temperature on growth, chemical composition and fatty acid composition of tropical Australian microalgae grown in batch cultures. Aquaculture 211, 195–214
Tao You and Stanley M. 2002, Barnett Effect of light quality on production of extracellular polysaccharides and growth rate of Porphyridium cruentum Biochemical Engineering Journal 19, 251-258
Thampson, Jr. G.A., 1996, Lipids and membrane function in green algae. Biochem. Biophys. Acta, 1302, 17-45
Tapie P. and Bernard A., 1987, Microalgae production: technical and economic evaluation. Biotechnol. bioeng. 32, 873-855
Xiaoling Miao, Qingyu Wu and Changyan Yang,2004, Fast pyrolysis of microalgae to produce renewable fuels. Journal of Analytical and Applied Pyrolysis, 71 855-863
Zhi-You Wen, Feng Chen, 2003, Heterotrophic production of eicosapentaenoic acidby microalgae, Biotechnol. adv. 21, 273-249
高宜廷,2002,新型光生化反應器之開發與其在單細胞藻類培養之應用，國立清華大學化學工程研究所碩士畢業論文。