本研究研發一種雙層奈米金以石墨烯薄膜為夾層之三維結構用於強化表面增強拉曼散射之生物感測器之性能。石墨烯薄膜材料因著其出色的物理及化學特性成為近年來在電子與光電元件領域極具發展潛力的材料，例如其具有高熱導性、高強度機械性質和高電子傳導特性…等。表面增強拉曼散射光譜近年來逐漸發展成分辨化學結構及未知樣品之有效工具，典型之表面增強拉曼散射是藉由電漿共振耦合引致極化效應所產生之現象，而製造大量拉曼增強效應之結構是目前所使用的主要方法，其中尺寸大於30奈米之奈米粒子或奈米結構陣列被大量的使用。此外，強烈的耦合效應通常只發生在幾個奈米的間距之間。
有鑑於以上之需求，我們合成尺寸約10奈米的金球並且使用厚度約0.34奈米的單層石墨烯薄膜分離兩層金奈米結構。利用這樣的結構我們可以進一步的增強表面增強拉曼散射的效果並且也可使整體結構尺寸更接近甚至小於10奈米，讓此結構更適合應用於生物量測的領域。
本研究建構兩種金屬結構系列：奈米金球 / 石墨烯薄膜 / 奈米金球 ( GNP / SLG / GNP ) 、 奈米金球 / 石墨烯薄膜 / 奈米島狀金 ( GNP / SLG / GNI )，主要差別在於第二層的材料製成。對於 GNP / SLG / GNI 系列的結構而言，因第二層奈米島狀金膜的覆蓋，使得訊號得以獲得一定程度的提升，但當將第二層材料換為 GNP / SLG / GNP 系列的金球時，因著hotspot區域的增加使得拉曼訊號得以獲得更進一步大幅度的增加，其訊號提昇倍率相較傳統金球陣列達到57倍的增強，並且可將待測之拉曼分子其拉曼光譜完整且清楚的呈現。

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