在此碩論中，我們建立了結合兩種互補成像模態的顯微影像技術，使用光譜域頻光學同調斷層掃描和虛擬偵測器光學解析度光聲顯微鏡實現顯微影像。所建立的顯微影像系統將能夠同時提供用於生物組織中的光學散射和光學吸收的體積顯微成像。在我們的系統中，光聲顯微鏡及光學同調斷層掃瞄共享光學掃描和傳送機制。此外，兩個系統的影像擷取可在單次掃描中同時完成。我們透過對微血管模型和洋蔥進行造影，驗證了這種新技術及顯微造影系統。本研究中的實驗證明了兩種系統的組合將如何提供對比的形態學信息。這將會是同時進行功能性和形態信息檢測的極具潛力的方法。

In this thesis, we built a novel dual-modal micro imaging system, combining two complementary imaging modalities, spectral domain optical coherence tomography and virtual detector laser scanning optical resolution photo-acoustic microscopy. This integration will be able to provide simultaneous volumetric microscopic imaging with optical scattering and optical contrast in biological tissue. In this integrated system, the optical scanning and delivery mechanism is shared between the two different imaging modalities. Furthermore, the image acquisition of the two systems in this thesis are done simultaneously in a single scan. A demonstration of this novel technique was shown through imaging both a microvascular tube phantom and an onion. The experiments in this study demonstrates how the combination of the two systems will provide contrasting morphological information. This would be a potential method for simultaneously detecting functional and morphological information.

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