產地是消費者選購茶葉考量的因素之一，進口茶葉假冒台灣茶葉事件層出不窮，為保障農民權益與提升消費者購買台灣茶葉信心，以科學方法輔助產源鑑定勢在必行。本研究使用高效液相層析儀及氣相層析質譜儀兩種儀器建立代謝物指紋圖譜，區別台灣魚池鄉、越南、印度、印尼、斯里蘭卡等五個不同產地的紅茶。高效液相層析儀之待測物為沒食子酸、5種兒茶素（兒茶素、表兒茶素、表沒食子兒茶素、表沒食子兒茶素沒食子酸酯、表兒茶素沒食子酸酯）及2種生物鹼（咖啡因、可可鹼）共8個次級代謝物，以此進行判別分析正確率為91.3%。使用氣相層析質譜儀進行之研究，應用代謝組學研究方法，待測物為以甲醇、水、氯仿混合溶液萃取分液後所得之所有極性代謝物（主要為初級代謝物）。根據判別分析結果提出以有機酸作為茶葉產源指標，以9種有機酸為變數進行判別分析的正確率為95.7%。本研究證實以代謝物指紋圖譜區別產地之可行性。

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