塑膠為主的海洋垃圾為日益嚴重的全球性環境污染問題，近年來許多研究顯示海洋中的塑膠在裂解後，會形成不同形狀的微形塑膠，數量龐大，對海洋以及水生生態造成重大影響。根據美國海洋暨大氣總署定義，微型塑膠是指長度小於5 mm的塑膠碎片或顆粒。
本研究分三部分。第一部份研究利用螢光呈色法，結合光學顯微鏡檢測日常商品中的微型塑膠，包含市售瓶裝水、食鹽、加水站與自來水，其結果可觀察到清楚易辨識的微型塑膠，多為纖維、顆粒及薄片等形狀。接著使用顯微共焦拉曼儀進一步鑑定出塑膠種類，其中以PP(聚丙烯)、PE(聚乙烯)等生活常見之塑膠為大宗。
國際間目前尚無人體的微型塑膠每日容許攝取量標準，與對人體危害性的結論，第二部分因此開始探討微型塑膠對生物的影響，進行大型水蚤(Daphnia magna) 的累積代謝實驗。同時也模擬真實環境的狀況，設計實驗參數，進行人工塑膠老化，並且使用顯微共焦拉曼儀和飛行時間二次離子質譜儀分析其差異。整體結果為微型塑膠並無明顯急毒性，且於一般狀況下沒有明顯累積現象，暴露後24小時，水蚤體內已無觀察到微型塑膠。少部分暴露於高濃度組別的水蚤(約5%)出現體內聚集現象，因腸道堵塞而導致死亡。此外經分析後結果，老化的塑膠以PVC(聚氯乙烯)表面性質差異最大。
第三部分則是進行微型塑膠吸附毒性物質實驗與生物急毒性測試，探討微型塑膠與環境中毒性物質間交互作用對生物的影響，初步結果顯示暴露於微型塑膠環境下的鄰苯二甲酸酯類DEHP及銅離子，對水蚤的毒性都有增加的趨勢，飛行時間二次離子質譜儀分析微型塑膠表面組成結果，說明電荷和凡得瓦力為主要機制。

Plastic in ocean is an increasingly problem of globally environmental pollution. In recent years, many studies have shown plastic in ocean usually degraded to different-shape micro plastics (also known as microplastics) after being cracked or decomposed. It has a major impact on the marine and aquatic ecology. The US Oceanic and Atmospheric Administration has defined microplastics as plastic fragments or granules with length less than 5 mm

This study is divided into three parts. The first part uses the fluorescent stainning method combined with an fluorescence mode microscope to investigate daily commodities, including bottled water, salt, and tap water. The results show that there are clearly identifiable microplastics, mostly in the shape of fibers, fragments and flakes. The confocal raman microscope was used to further identify the plastic type. The bulk of these particals are PP(polypropylene) and PE (polyethylene).

Nowadays, there is no standard of daily intake of microplastics, and clear conclusion about its harm to human in the world. The second part therefore began to explore the effects of microplastics on organisms. We conducted bioaccumulation metabolism experiment on Daphnia magna, and performed artificial plastic aging process by simulating the condition of real environment. The overall result was that microplastics had no significantly acute toxicity, and there was no obvious accumulation phenomenon under normal condition. After exposure, no microplastics were observed in the Daphnia magna in 24 hours. A small percentage of Daphnia magna (about 5%) exposed to high-concentrationed groups showed aggregation in the body and died due to intestinal obstuction. In addition, aged PVC (polyvinyl chloride) have the largest difference in surface properties.

The third part is to carry out microplastics adsorption experiments and biological acute toxicity tests to explore the effects of interaction between microplastics and harmful toxicants in the environment. The preliminary results show that phthalate DEHP and copper ions exposed to microplastic environment had an increasing tendency toward biotoxicity on Daphnia magna. The results of surface composition analysis by TOF-SIMS indicate that charge attraction and Van der Waals force as the main adsorption mechanisms.

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