本文主要探討纖維母細胞的遷移，使用兔子關節滑膜的纖維母細胞，HIG-82做為培養的細胞株，基材為矽基板，先以黃光利用黃光微影技術定義出微米柱區域，再配合奈米銀粒子蝕刻，製作出奈米結構週期結構的矽基材表面，最後，以分子氣相沈積方式，在已蝕刻的奈米表面鍍上FDTS疏水層，在設計上，微米柱間距並不等向，列間距為20 到60微米，行間距則是固定為200微米。
12小時的培養後，可以發現纖維母細胞(HIG-82)可以不會跨越大於45微米的間距，但是對於45微米的間距卻是可以跨越，並連接在一起，形成由細胞構成的線，這條線的長度最長可以到達1000微米。此外，在短時間的觀察中，在微米柱上的細胞會伸出觸手(fliopodia)感測周圍環境，並在幾個小時內連接在一起。這個結果可以幫助我們了解細胞的遷移與生長，微奈米結構的設計更可以讓細胞生長接近生理的狀況。

關鍵字:纖維母細胞，微奈米結構

In this study, the migration of HIG-82 fibroblasts can be controlled by nano/mirco patterned oxidized silicon surface in vitro. The microposts were defined through photolithography and silver nanoparticles assisted etching was used to produce the nanostuctures. After that, the hydrophobic chemical was coated on the surface of nanostructures to form hydrophilic/hydrophobic composite structures. In order to investigate cell anisotropic migration, the spacing of micropost between columns and rows was designed to have various distances. The distances between columns ranged from 20 to 60 μm while the distance between rows was kept constant at 200 μm. In this research, we discovered that when the distances of micropost increase, the cells would hardly migrate from one post to another. If the distance of micropost is over than 45 μm, the cells almost cannot cross the gap. The cell can form a long line which is up to 1000 μm in 12 hours while the gap is less than 45μm. The process of cell fusing is also investigated. Cells expanded fliopodia to detect environment and connected to each other in few hours. In conclusion, this study implicates that the migration and exploration of fibroblasts can be observed on the patterned oxidized silicon surface. The cells growth direction can also be controlled using this kind of patterns.

Keywords: fibroblast, nanostructure, FDTS

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