格蘭氏陰性菌(Gram negative bacteria)細胞壁上的酯多醣(lipopolysaccharide)，又稱為內毒素(endotoxin)，相對於蛋白質而言是一種熱源(pyrogen)以及非常穩定的分子，在極端的溫度以及酸鹼值下仍具有生物活性，此外，酯多醣並不能像蛋白質一樣容易用烘乾或是加熱的方法去除乾淨，而內毒素進入動物體內會造成嚴重的敗血症(septicaemia)，甚至使病患在幾小時內喪命。 為了發展出細菌和內毒素檢測試劑，本篇研究利用嗜甲醇酵母菌(Pichia pastoris)成功表現出重組蛋白質ALT2，ALT2由胺基端的米根黴菌澱粉吸附區域(Rhizopus oryzae starch-binding domain, RoSBD)、米根黴菌葡萄糖水解酵素連結片段(Rhizopus oryzae glucoamylase linker, Linker)以及與羧基端的台灣種類馬蹄蟹血液中的血漿凝集素2(Taiwanese Tachypleus tridentatus , TPL2)組成。先前的研究證明，TPL2對於內毒素的O-antigen部位具有高度結合力。本篇研究發現連結片段不只將RoSBD和TPL2區隔開，而且解決的重組蛋白質會被蛋白質水解脢水解的瓶頸。此外，重組蛋白質ALT2中的澱粉吸附區域能夠使重組蛋白質經由吸附澱粉的方式快速純化，利用澱粉作為蛋白質純化吸附材質，不僅可以降低純化成本，而且操作上安全又方便。更近一步研究發現，重組蛋白質ALT2中的TPL2仍然具有辨識格蘭氏陰性菌E. coli的活性。因此，重組蛋白質ALT2不但可以降低工業上生產成本，未來也具有潛力發展成為診斷不同種類細菌或內毒素的檢測試劑。

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