本研究的目的，是解決電子顯微鏡應用於活體細胞或軟物質材料遇到的挑戰-潮濕樣品中的水分會被顯微鏡真空系統抽乾。利用微機電製程的濕室環境元件, 此元件外觀邊長近似2.5mm的正方型，中間利用蝕刻製程的氮化矽薄膜(50nm)為主要的細胞培養及觀測區間。我們將細胞分為 1)脫水乾燥2)潮濕及3)流體循環做電子顯微鏡觀測。並在後續章節描述設計原理與實驗操作方法。
乾燥脫水主要是將細胞製作成標本，保留原本結構;潮濕狀態機制，是利用環境濕室元件的底座(Outframe)配合設計的金屬載具盒，增加濕室元件內的容量以用來做長時間細胞培養與觀測。本實驗目標主要是以流體循環的方式將活體細胞成功地培養在自行設計的元件上，考量到細胞取得方便及環境的承受度，我們所使用的是人類子宮頸癌細胞(HeLa cell);培養液是用Dulbecco改良培養基(D-MEM)、胎牛血清(FBS)及青黴素/鏈黴素(Pen/Strep)所配製而成的。將細胞和細胞培養液以1:3的比例滴入元件中，並在電子顯微鏡中做長時間觀測,比較癌細胞乾操、含水與流體置換狀態的影像，並進一步觀測細胞分裂、胞吞等生物作用。

The goal in this research is to solve the challenge that using electron microscope on the living cell or soft matter materials-the moisture in the wet sample will be dried out by the microscope's vacuum system. We use MEMS manufacture's wet room environment component which the exterior appearance is approximately 2.5mm square size and use Silicon Nitride Films (50nm) which is etched in the middle as the main cell culturing and observation zone then divide cell into 1)dehydration, 2)humidification, and 3)fluid circulation for electron microscope observation. In the further chapter, we will describe the theory of design and the operation of the experiment.
The first one, dehydration, make cell specimens to maintain the original construction. Second, humidification, use metal holder box that designed to match the out-frame of wet room environment component to increase the capacity of wet room for the long-time cell culture and observation. The objective in this experiment is to culture living cell successfully on the self-design component by fluid circulation way. Considering the convenience and tolerance to environment, we use HeLa cell. And the medium is mixed by D-MEM, FBS, and Pen/Strep.
Adding in the component, in the ratio of 1:3 between cell and the medium, and observing for a long time under the electron microscope, we compare the images of dehydration, humidification, and fluid circulation cancerous cell; moreover, observe the cell division, endocytosis, etc.

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