在人體內神經元(neuron)是一種高度分化的細胞，具有許多特化的次細胞結構。而各個次細胞結構中的蛋白質分析，一直是科學家們致力研究的課題。其中神經軸突是神經細胞中最長的神經纖維，負責著訊號傳導的工作。然而，在目前的文獻中並無好的方法學能夠收集大量且純度高的神經軸突蛋白質，所以在本論文嘗試結合微機電系統與神經細胞培養，製作細胞生長的微接觸壓印刻痕玻片，以分離、純化、收集神經軸突與生長錐等次細胞結構以進行蛋白質分析。在研究中，我們發現以往以人手操作的收集方式，不但穩定性不佳且收集時程較長，因此我們根據玻片斷裂測試結果設計刻痕玻片斷裂操作台以提昇收集過程的穩定度與節省收集時程。除此之外，根據神經軸突蛋白質分析的結果顯示。此方法學所收集的神經軸突蛋白質並無遭受細胞體蛋白質污染。而在與細胞體蛋白質比較後，發現神經軸突蛋白質具有不同的組成與不同的轉譯後修飾機制(post-translational modification)。利用此方法學，未來可望能夠對神經軸突蛋白質有更加深入的探討與分析，進而對於神經軸突的引導(guidance)，再生(regeneration)等研究課題有著重要的貢獻。

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