現在奈米科技中利用對環境無害且簡單、方便和室溫下的製程來合成奈米材料是一個可靠且相當重要的一個趨勢。本實驗室之前利用CFB系統可以合成奈米銀顆粒和奈米銀線，不過，還是無法分離二者。本篇論文在CFB系統上改變硝酸銀濃度進而使產物只有奈米銀顆粒，並利用雷射散射粒徑分析儀來測粒徑和顆粒分佈，結果發現在作用濃度為0.7 mM，其粒徑大小及分佈較為均勻約為25.8 nm。在CFB保存實驗中，取不同保存時間的CFB，可以發現粒徑和顆粒數都呈穩定的狀況，由此可見CFB可以長時間保存。CFB系統中粒徑會隨著時間變化而增大，因此在該時間點將奈米銀顆粒和CFB分離並利用超音波震盪來分散聚集的顆粒，並藉此來維持該時間點的粒徑。
在顆粒應用上，本實驗做了細菌的測試，我們選用了Deinococcus radiodurans TSB、Acetobacter aceti、Ｅ. Coli ，實驗發現在低濃度時，發現菌會被抑制生長，將濃度依序提高，可使菌的存活率降低至0.01％，結果顯示確實是有抗菌的效果。總結來說，本實驗利用一個對環境無害、簡單且有效率的新生物製程來合成奈米銀顆粒，在將來，或許可以利用該製程來製備新的抑菌材料，或是其他一些相關的應用。

The development of reliable, eco-friendly process for the synthesis of nanomaterials is an important aspect of nanotechnology today. In the past, by using Deinococcus radiodurans TSB CFB system, we’re been able to produce silver nano-structure including particles and wires. By controlling the concentration of silver ion, we’ve be able to produce silver nanoparticles only. Using Zeta sizer to assay the size and distribution of nanoparticles. Under 0.7 mM of silver nitrite, the silver nanoparticles have uniformed size without significant variation in size. At different stocks of CFB shows that silver nanoparticle size and particle number do not have specific variation.
In applications , the antimicrobial activity of silver nanoparticles, we choose Deinococcus radiodurans TSB、Acetobacter aceti、Ｅ. Coli . These particles were shown to be an effective bactericide with different of concentrations of silver nanoparticles .These nontoxic nanoparticles, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal nanomaterials or other applications.

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