研究生: |
宋榮城 |
---|---|
論文名稱: |
硫酸鹽還原菌gigas導致鐵的厭氧腐蝕下的3維x光斷層掃瞄重建 The anaerobic biocorrosion of iron by sulphate-reducing bacteria gigas with 3-D reconstruction of x-ray tomography at 60nm |
指導教授: |
吳文桂
湯茂竹 |
口試委員: | |
學位類別: |
碩士 Master |
系所名稱: |
生命科學暨醫學院 - 生物資訊與結構生物研究所 Institute of Bioinformatics and Structural Biology |
論文出版年: | 2007 |
畢業學年度: | 96 |
語文別: | 中文 |
論文頁數: | 80 |
中文關鍵詞: | 厭氧腐蝕 、x光斷層掃瞄 |
相關次數: | 點閱:3 下載:0 |
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脫硫弧菌屬的硫酸鹽還原菌是微生物中對腐蝕影響最大、研究最多、人們最為重視的厭氧腐蝕成因,此細菌在材料上產生的腐蝕現象,對經濟上造成了相當大的損失。從過去的研究發現硫酸鹽還原菌可使碳鋼造成腐蝕,並生成黑色硫化鐵的腐蝕產物。而本論文是使用X光顯微鏡作為實驗工具,將鐵試片以及不鏽鋼試片浸泡在培養好的硫酸鹽還原菌菌液中根據不同的參數條件下進行實驗,硬X光顯微鏡由於所使用X光波長短,理論上具備高空間解析度,能幫助我們觀察樣品微觀結構;對於樣品有高穿透力,能進行非破壞性偵測使樣品不被破壞;使用同步輻射光源優異的特性(高強度、高準直性及可以得到連續可調波長的光)能把它應用在X光斷層掃描術作為光源,應用同步輻射光源做出的樣品3維重建影像的解析度已達奈米等級;相較於電子顯微鏡,對於樣品的製備更為容易,不需要過多的染色、切片,之後再將腐蝕材料樣品進行2維顯微成像以及3維斷層掃描成像,同時利用影像處理軟體—Amira進行影像分析與影像重建,將不同的腐蝕材料樣品做統計分析比較,幫助我們判斷硫酸鹽還原菌在不同的環境參數下,腐蝕材料樣品經過重建後的影像有何特性?我們就可以根據重建完的影像討論出硫酸鹽還原菌在腐蝕過程中所扮演的角色,期望能發現抑制及預防細菌腐蝕的條件!
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