蘇力桿菌是一種能形成孢子的革蘭氏陽性(Gram positive)好氣性桿菌，菌體兩端鈍圓，有些種類菌體周生鞭毛，具有運動能力，然而有些菌種不生鞭毛，因此不具運動能力。蘇力桿菌通常2~8個呈鏈狀，廣泛地存在於土壤之中[Yu, 1990]。到目前為止，至少已知有38種此類菌株可產生出殺蟲性毒素。我們的實驗目的就是在探討運動性在蘇力菌生理上扮演的角色，在實驗過程中，我們定義出一個物理量〝平均運動速率〞(average motile rate)，來表示菌體在生長過程中的運動情形。
首先，我們藉由觀察平均運動速率與菌體量、蘇力菌素產量隨環境因子的變化趨勢，來探討平均運動速率與蘇力菌生理之間的關連性。根據實驗結果，可得到以下結論：

(1) 作為觀察菌體生長的新資訊

(2) 預測蘇力菌素的產量

其次我們想要進一步瞭解，不同的平均運動速率時期，對菌體而言是否代表了不同的生理時期。因此我們選擇了運動速率的高峰期、遞減期與平穩期，將通氣量、溫度與酸鹼值做一為時二十分鐘的脈衝變化，根據實驗結果可得到結論如下：

(1) 對蘇力菌而言，當批次系統在醱酵前期受到一定程度的干擾時，對於產物的生成都是不利的

(2) 當平均運動速率愈高時，環境的改變對菌體的生理影響愈大

(3) 傳統的指數生長期可藉由菌體平均運動速率，再區分成不同生理活性時期

Bacillus thuringiensis is an aerobic, Gram positive bacterium which produce spores when the environment goes against its growth. Some sorts of Bacillus thuringiensis have surrounding flagella while the others not. Those who possess flagella have the ability to swim. Bacillus thuringiensis cells usually gather in the form of chains while each chain is composed of 2-8 cells; they are frequently found in the soil. At least 38 strains are known to produce insecticides. This work is to study the role of the motility which plays in the physiology of Bacillus thuringiensis. We define a term "Average Motile Rate" (AMR) to describe the motivation of cells during the cultivation.
The changing tendencies of AMR, biomass and yield of thuringiensin which respect to some environmental factors are observed to investigate the relationship between the AMR and the physiology of Bacillus thuringiensis. The results showed that:

(1) The AMR gave new information of cell growth

(2) The AMR could aid prediction of the yield of thuringiensin

Also, it is desired to know whether each AMR period indicates a

corresponding physiological period of Bacillus thuringiensis or not. Pulses of aeration rate, temperature and pH value which last 20 minutes were carried out during the peak period, the decay period and the stable period of the AMR. We then draw following conclusions:

(1) It was harmful to the production of thuringiensin when the cultivation was disturbed during its early fermentation stage

(2) The influence upon the cell physiology increased with higher values of AMR

(3) The conventional exponential phase could be further split into two physiological active stages according to the AMR

1. Alweiss, B., Dostal J., Carey K. E., Edwards T. F. and Freter R. "The
role of chemotaxis in the ecology of bacterial pathogens of mucosal
surface" Nature, V.266, 448, 1977
2. Andreev J., Dibrov P. A. and Braun S. "Motility and chemotaxis in
Bacillus sphaericus." FEBS Letters, V.349, 411-415, 1994
3. Andrews, R. E., Faust, R. M., Wabiko, H., Raymond, K. C. and Bulla,
J. L. A. "The biotechnology of Bacillus thuringiensis." CRC Critical Rev.
Biotechnol., V.6, 163-232, 1987
4. Armitage, J. P., Gallagher A. and Johnston A. W. B. "Comparison of the
chemotactic behavior of Rhizobium leguminosarum with and without
the Nodulation plasmid." Molecular Microbiol., V.2, 743, 1988
5. Barjac, H. de, Burgerjon A. and Bonnefoi A. "The production of heat-
stable toxin by nine serotypes of Bacillus thuringiensis." J. Invertebr.
Pathol., V.4, 537-538, 1966
6. Branimir Z. and Franio K. : Description of the invention rekation to
patent 664531 (in Russian), 1979
7. Bret R. Phillips, John A. Quinn and Howard Goldfine "Random
motility of swimming bacteria : single cells compared to cell
populations." AICHE Journal, V.40, No.2, 334-348
8. Briggs, J. D. "Reduction of adult house fly emergence by the effect of
Bacillus spp. on the development of immature forms." J. Insect Pathol.,
V.2, 418-432, 1960
9. Burgerjon, A. and H. de Barjac "Nouvelles donnees sur le role de la
toxine soluble thermostable produite par Bacillus thuringiensis." C. R.
Acad. Sci. Paris Serie D, V.251, 911-912, 1960
10. Burgerjon, A. and H. de Barjac "Essais preliminaires sur le role
insecticide de la toxine thermostable produite par Bacillus
thuringiensis Berliner." Proc. Int'l. Congr. Entomol. Vienna,
835-839, 1960
11. Burgerjon, A. and H. de Barjac "Etude de la toxine thermostable chez
differentes souches de Bacillus thuringiensis Berliner." Coll. Int.
Pathol. Insect. Lutte Microbienne Paris, 1962, Entomophaga (hors
series), V.2, 222-226, 1964
12. Burgerjon, A. and H. de Barjac "Another serotype (4: 4a, 4c) of
Bacillus thuringiensis which produces thermostable toxin." J.
Invertebr. Pathol., V.9, 574-577,1967
13. Burges, H. D. "Progress in the microbial control of pets, 1970-80" in
Microbial control of pets and plant diseases 1970-1980, 1-6,
Academic Press, London, 1981
14. Campbell, D. P., Dieball, D. E. and Brackett, J. M. "Rapid HPLC
assay for the β-exotoxin of Bacillus thuringiensis." J. Agric. Food
Chem., V.35, 156-158, 1987
15. Cantwell G. E., Dougherty E. and Cantelo W. W. "Activity of the
β-exotoxin of Bacillus thuringiensis var. thuringiensis against the
Colorado potato beetle and the ames test." Enviro. Entomol., V.12,
No.5, 1424-1427, 1983
16. Cantwell, G. H., Heimpel A. M. and Thompson M. J. "The
production of an exotoxin by various crystal forming bacteria related
to Bacillus thuringiensis var. thuringiensis Berliner." J. Insect
Pathol., V.6, 466-480, 1964
17. Charles D. Amsler, Myungsun Cho, and Philip Matsumura "Multiple
Factors Underlying the Maximum Motility of Escherichia coli as
Cultures Enter Post-Exponential Growth," Journal of Bacteriology.,
V.175, No.19, 6238-6244, 1993
18. Chet, I.,P. Asketh, and R. Mitchell,"Repulsion of Bacteria from
Marine Surfaces,"Appl. Microbiol.,30, 1043 (1975)
19. Daniel B. Mirel, Veronica M. Lustre and Michael J. Chamberlin "An
operon of Bacillus subtilis motility genes transcribed by the sD form
of RNA polymerase." Journal of Bacteriology, V.174, No.13, 4107-4204,
1992
20. Dulmage H. T. "Insecticidal activity of isolates of Bacillus
thuringiensis and their potential for pest control." In Microbial
Control of Pests and Plant Diseases 1970-1980, 193-222, Academic
Press, London, 1981
21. Dunn, P. H. "Control of house flies in bovine feces by a feed additive
containing Bacillus thuringiensis var. thuringiensis Berliner." J.
Insect Pathol., V.2, 13-16, 1960
22. Fast, P. G. "The crystal toxin of Bacillus thuringiensis." In Microbial
Control of Pests and Plant Diseases 1970-1980, 223-248, Academic
Press, London, 1981
23. Freter, R., O'Brien P. C. M. and Macsai M. S. "Role of chemotaxis in
the association of motile bacteria with intestinal Mucosa : in vivo
studies." Infec. Immun., V.34, 234, 1981
24. Gilman, M. Z. and Chamberlin M. J. "Developmental and genetic
regulation of Bacillus subtilis genes transcribed by s28-RNA
polymerase." Cell, V.35, 285-293,1983
25. Gilman, M. Z., Glenn J. S., Singer V. L. and Chamberlin M. J.
"Isolation of sigma-28-specific promoters from Bacillus subtilis
DNA." Gene, V.32, 11-20, 1984
26. Gulash, M., Ames P., Larosiliere R. C. and Bergman K. "Rhizobia
are attacted to localized sites on legime roots." Appl. Env.
Microbiol., V.48, 149, 1984
27. Hall, I. M. and Arakawa K. Y. "The susceptibility of housefly Musca
domestica Linnaeus to Bacillus thuringiensis var. thuringiensis
Berliner." J. Insect Pathol., V.1, 351-355, 1959
28. Hall, R. L. "Amoeboid movement as a correlated walk." J. Math.
Biol., V.4, 327, 1977
29. Heimpel, A. M. "A critical review of Bacillus thuringiensis var.
thuringiensis Berliner and other crystalliferous bacteria." Ann. Rev.
Entomol., V.12, 287-322, 1967
30. Helmann, J. D. "Alternative sigma factors and the regulation of
flagellar gene expression." Mol. Microbiol., V.5, 2875-2882, 1991
31. Holmberg, A., Sievanen, R. and Carlberg, G. "Fermentation of
Bacillus thuringiensis for exotoxin production process analysis
study." Biotechnol. Bioeng., V.22 1707-1724, 1980
32. Ignatenko Yu. N., Sakharova Z. V., Khovrychev M. P. and Shevtsov
V. V. "Effect of temperature and aeration on growth and spore
formation in Bacillus thuringiensis." Microbiologiya, V.52, No.5,
716-718, 1983
33. Ishiwata, S. "On a kind of severe flacherie (sotto disease)." Dainihon
Sanshi kaiho, V.9, 1-15, 1901
34. Jerald, S. F., Jewel, P. and Leo, K. "Bacillus thuringiensis insects and
beyond." Bio/Tech., V.10, 271-275, 1992
35. Kennedy, M. J. amd Lawless J. G. "Role of chemotaxis in the
ecology of denitrifiers." Appl. Env. Microbiol., V.49, 109, 1985
36. Lambert, B. and Peferoen, M. "Insecticidal promise of Bacillus
thuringiensis." BioSci., V.42, 112-121,1992
37. Levinson, B., Kasyan, K. J. and Chiu, S. S. "Identification of
β-exotoxin production, plasmids encoding β-exotoxin and a new
exotoxin in Bacillus thuringiensis by HPLC." J. Bacteriol., V.172,
3172-3179, 1990
38. Marec, F., Matha, V. and Weiser J., "Analysis of the Genotoxic
Activity of Bacillus thuringiensisβ-exotoxin by means of
Drosophila Wing Spot Test." J. Inverte. Pathol., V.53, 347-353, 1989
39. Markl, H. and Bronnenmeier, R. "Mechanical stress and microbial
production." in Biotechnology, V.2, Chapter 18, 369-392, 1985
Paige, M. R. and Cooper, R. D. "Scale-up of beta exotoxin production in
fed-batch Bacillus thuringiensis fermentation." Eur. Congr. Biotechnol.,
5th meet., 146-159, 1990
40. Marquez, L. M., Helmann J. D., Ferrari E., Parker H. M., Ordal G.
W. and Chamberlin M. J. "Studies of sD-dependent functions in
Bacillus subtilis." J. Bacteriol., V.172, 3435-3443, 1990
41. McConnel, E. and Richards A. G. "The production by Bacillus
thuringiensis Berliner of heat-stable substance toxic for insect."
Canad. J. Microbiol., V.5, 161-168, 1959
42. Mohd-Salleh, M. B., Beegle C. C. and Lewis L. C. "Fermentation
media and production of exotoxin by three varieties of Bacillus
thuringiensis." J. Inverte. Pathol., V.35, No.1, 75-83, 1980
43. Mummigatti, S. G., and Raghunathan A. N. "Production of Bacillus
thuringiensis var. kurstaki by three different methods and its relative
toxicity to Bombyx Mori." J. Inverte. Pathol., V.54, No.4, 272-3276,
1991
44. Ohba, M., Tantichodok A. and Aizawa K., "Production of heat-stable
exotoxin by Bacillus thuringiensis and related bacteria." J. Invert.
Pathol., V.38, 26-32, 1981
45. Packer, H. L., Keshavarz-Moore E., Lilly M. D. and Thomas, C. R.,
"Estimation of cell volume and biomass of Penicillium chrysogenum
using image analysis." Biotechnol. Bioeng., V.39, 384-391, 1992
46. Othmer, H. G., Dunbar S. R. and Alt W. "Models of dispersal in
biological systems." J. Math. Biol., V.70, 133, 1972
47. Rodriguezpadilla, C., Galanwong, L., de Barjac, H., Romancalderon,
E., Tamezguerra, R. and Dulmage, H. "Bacillus thuringiensis
neoleonensis serotype H-24, a new subsp. which produces a
triangular crystal." J. Inverte. Pathol., V.56, 280-282, 1990
48. Rowe, G. E. and Margaritis, A. "Bioprocess developments in the
production of bioinsecticides by Bacillus thuringiensis." CRC
Critical Rev. Biotechnol., V.6, 87-127, 1987
49. Schugerl, K. "Comparison of the performances of stirred tank and
airlift tower loop reactors." J. Biotechnol., V.13, 251-256, 1990
50. Sebesta, K., Farkas, J. and Horska, K. "Thuringiensin, the β-
exotoxin of Bacillus thuringiensis." In Microbial control of pests and
plants diseases 1970-1980, 249-281, Academic Press, London, 1981
51. Stanton, T. B. and Savage D. C. "Colonization of gnotobiotic mice by
Roseburia cecicola, a motile, obligately anaerobic bacterium from
murine ceca." V.45, 1677, 1983
52. Thomas C. R. "Image analysis : putting filamentous microorganisms
in the picture." BITECH, V.10, 343-348, 1992
53. Vandekar, M. and Dulmage H. T. "Guidelines for production of
Bacillus thuringiensis H-14." Published by UNDP/World Bank/WHO
Special Programme for Research and Training in Tropical Diseases.
Geneva, Switzerland, 1983
54. van Suijdam J. C. and Metz, B. "Influence of engineering variables
upon the morphology of filamentous molds" Biotechnol. Bioeng.,
V.23, 111-148, 1980
55. Yu, Z. N. "Bacillus thuringiensis", Science Publisher, Peking, 1990
56. 李國鏞，〝微生物學〞，華香出版社，1991
57. 吳美貌，〝蘇力菌素的醱酵產製、分離純化與生物檢定〞，大葉
工學院食品工程研究所碩士論文，1993
58. 吳美貌、曾耀銘、徐泰浩，〝蘇力菌素高產率醱酵暨高效液相層
析全程偵測探討〞，技術學刊，第八卷第二期，223-230，1993
59. 連國珍，〝數位影像處理〞，儒林圖書有限公司，1992
60. 黃定國，〝動態調整通氣量以控制蘇力菌醱酵程序之起泡現象〞，
清華大學化學工研究所碩士論文初審，1998
61. 陳斐筠，〝影像分析在菌體測量上之應用〞，清華大學化學工研
究所碩士論文，1998
62. 熊定宇，〝具網狀導管氣舉式反應器之設計及其在蘇力菌醱酵之
應用〞，清華大學化學工程研究所博士論文，1995
63. 鍾建中，〝網狀內管氣舉式反應器在蘇力菌素生產上之應用〞，
清華大學化學工程研究所博士論文，1994
64. 簡道南，〝利用微生物生產農業用殺蟲劑-蘇力菌結晶蛋白之產
程開發〞，應用化學研討會，63-74，1992
65. 蘇遠志、黃文瑛，〝蘇力菌孢子與毒素結晶之製劑及其應用〞，
中國農業化學會誌，第三十卷第四期，569-581，1992