多壁奈米碳管之於大腸桿菌之影響研究｜國立清華大學博碩士論文庫

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研究生：	許惠珍
論文名稱：	多壁奈米碳管之於大腸桿菌之影響研究 The Effects of Multi-walled Carbon Nanotubes on Escherichia coli
指導教授：	戴念華宋信文
口試委員:
學位類別：	碩士 Master
系所名稱：	工學院 - 材料科學工程學系 Materials Science and Engineering
論文出版年：	2008
畢業學年度：	96
語文別：	中文
論文頁數：	86
中文關鍵詞：	多壁奈米碳管、抗菌
外文關鍵詞：	multi-walled carbon nanotubes, antimicrobial
相關次數：	點閱：2 下載：0
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　　本研究首先測試三種不同形式之多壁奈米碳管（原始、純化和官能基化多壁奈米碳管）對典型微生物（大腸桿菌）的影響。經由掃描式電子顯微鏡（SEM）及螢光顯微鏡分析結果，是由於菌體與多壁奈米碳管的直接接觸造成其菌體外膜損傷而導致死亡，尤其在培養液中擁有極佳分散性之官能基化多壁奈米碳管能持續性地抑制細菌的存活率。
　　接著進一步利用化學氣相沉積法（快速升降溫系統）合成垂直成長在玻璃基板上的多壁奈米碳管叢（MWCNT forest）以探討其一維結構如何影響微生物。MWCNT forest與大腸桿菌培養24小時之實驗結果藉由場發射顯微鏡的觀察，可發現大部分附著在MWCNT forest上的大腸桿菌並無法維持外膜的形狀，此結果提供了一個有效的抗菌之應用。

Abstract ……………………………………………………………i
摘要 …………………………………………………………………ii
誌謝 …………………………………………………………………iii
總目錄 ………………………………………………………………v
表索引 ………………………………………………………………vii
圖索引 ………………………………………………………………viii
Chapter 1     緒論
 1.1    奈米碳管的簡介 …………………………………………1
   1.1.1    奈米碳管的起源 ……………………………………1
   1.1.2    奈米碳管的結構 ……………………………………2
   1.1.3    奈米碳管在醫療診斷、組織工程上之應用 ………4
   1.1.4    奈米碳管之抗菌活性 ………………………………8
 1.2    細菌之簡介 ………………………………………………10
   1.2.1    細菌型態 ……………………………………………10
   1.2.2    細菌的分類 …………………………………………12
   1.2.3    細菌的生長曲線及生長需求 ………………………13
 1.3    研究動機 …………………………………………………16
 1.4    實驗規劃 …………………………………………………17
Chapter 2 多壁奈米碳管(MWCNTs)的製備 ………………………20
 2.1    實驗動機 …………………………………………………20
 2.2    絨球狀之多壁奈米碳管之合成 …………………………20
   2.2.1    水平式懸浮觸媒化學氣相沉積法 …………………20
   2.2.2    多壁奈米碳管合成實驗步驟 ………………………22
 2.3    原始、純化和官能基化多壁奈米碳管粉末之製備 ……24
   2.3.1    絨球狀多壁奈米碳管之粉碎 ………………………24
   2.3.2    原始多壁奈米碳管粉末之純化 ……………………24
   2.3.3    原始多壁奈米碳管粉末之官能基化 ………………25
 2.4    多壁奈米碳管溶液之製備 ………………………………26
 2.5    原始、純化和官能基化多壁奈米碳管之形貌與性質
        分析結果 …………………………………………………27
   2.5.1    場發射電子顯微鏡之觀察結果 ……………………27
   2.5.2    拉曼光譜儀之分析結果 ……………………………28
   2.5.3    傅利葉轉換紅外線光譜儀之分析結果 ……………29
Chapter 3     原始、純化及官能基化多壁奈米碳管於大腸桿菌
              之抑菌評估 ………………………………………34
 3.1    實驗動機 …………………………………………………34
 3.2    細菌的培養 ………………………………………………34
   3.2.1    單一菌落之分離 ……………………………………34
   3.2.2    過夜菌液之培養 ……………………………………35
   3.2.3    Escherichia coli DH5α生長曲線的繪製…………35
 3.3    多壁奈米碳管抗菌實驗方法及步驟 ……………………37
   3.3.1    定性實驗之方法與流程 ……………………………37
   3.3.2    E. coli死亡率（Loss of Viability）之定量實
            驗方法 ………………………………………………42
 3.4    結果與討論 ………………………………………………43
   3.4.1    試樣處理過程之觀察 ………………………………43
   3.4.2    PI / DAPI染色之螢光分析結果……………………44
   3.4.3    場發射掃描式電子顯微鏡結果分析結果 …………46
   3.4.4    Loss of Viability分析結果………………………48
Chapter 4 MWCNT forest對Escherichia coli DH5α的影響……61
 4.1    實驗動機 …………………………………………………61
 4.2    MWCNT forest的製備 ……………………………………61
   4.2.1    MWCNT forest之分析結果 …………………………62
 4.3    MWCNT forest對Escherichia coli DH5α之傷害性
        測試 ………………………………………………………64
   4.3.1    實驗方法與步驟 ……………………………………64
4.4    結果與討論…………………………………………………64
Chapter 5 結論 ……………………………………………………73
Chapter 6 參考文獻 ………………………………………………74
附錄A、PI/DAPI螢光染劑與三種形式多壁奈米碳管之關係探討
       ………………………………………………………………78
附錄B、多壁奈米碳管於E. coli外膜之傷害程度探討 …………85

                                

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