在本研究中利用金導線陣列製作新穎之生物感測器，藉由量測肝功能重要指標-人類血清蛋白 (human serum albumin, HSA) ，期待未來整合於生物晶片中，用來即時檢測肝功能健康狀況。
在本研究中生物感測器製備方式，首先利用半導體製程製備生物感測器，其金電極間玻璃區域，以3-氨丙基三乙氧基矽烷 (3-aminopropyltriethoxysilane, APTES) 進行表面改質，使玻璃表面具有生物相容性後，依序固定上人類血清蛋白抗體 (anti-human serum albumin, AHSA) 、脫脂奶粉阻斷劑，以作為待測物人類血清蛋白 (HSA) 之接合與檢測。
在人類血清蛋白之感測與定量分析上，乃利用電化學阻抗譜 (Electrochemical Impedance Spectroscopy, EIS) ，在交流電下，直接量測接合於相鄰兩金電極間玻璃上之人類血清蛋白阻抗特性，藉由不同濃度貢獻之電容性阻抗變化量，定量分析人類血清蛋白之濃度。結果發現，當人類血清蛋白濃度越高，其貢獻的阻抗越大，且阻抗變化與人類血清蛋白濃度之對數 (log) 數值間呈線性關係；除此之外，此電化學阻抗式人類血清蛋白生物感測器，靈敏度可到達2□10-4 mg/ml。
本研究利用電化學阻抗式機制，用以定量檢測人類血清蛋白濃度，並成功證明此機制之可行性，且在感測器僅需利用單層黃光製程製作金電極陣列，並可直接製作於生物晶片上。因具備感測過程便利、易整合於生物晶片、分析結果可即時輸出、低成本等優點，對肝功能檢測上有極大應用價值。

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