早期利用抗體抗原法或是螢光蛋白雖然可以偵測核膜構形，但是對於正常核纖層蛋白與變異核纖層蛋白間關係仍未知。本實驗中，我們使用兩種新穎之螢光標記探針來觀察不同轉譯後修飾情況下正常與變異核纖層蛋白間之交互作用造成之核膜構形變化。研究結果表明，正常核纖層蛋白將受變異核纖層蛋白濃度影響，進而使核膜產生聚集或是皺褶之構形變化。此外，我們也探討了變異核纖層蛋白進行轉譯後修飾之核層形態的變化。我們發現法尼基化的變異核纖層蛋白可以在5-7小時內恢復正常核膜形狀，但是在非法尼基化的變異核纖層蛋白實驗中則表現出不同結果，即使追蹤至第24小時，非法尼基化的變異核纖層蛋白仍有聚集於核周圍之現象。
我們相信，透過了解核纖層蛋白之合成過程以及不同轉譯後修飾的形態變化，將會對相關的疾病的治療或是藥物的發展有非常大的幫助。

　　Although nuclear lamina morphology can be clearly visualized using Lamin-specific antibodies and fluorescent proteins, it has remained unclear how the normal lamina morphology would respond to the mutant preLamin in living cells. In this study, we used a pair of new fluorogenic labeling probes to elucidate the morphological interplay between mature and mutant Lamin A under two different posttranslational processing controls. Our results reveal that the shape of mature Lamin can be altered by the mutant preLamin and that this alteration is progressive, cumulative and due to a concentration-dependent dominant-negative effect of preLamin. The second set of our experiments explored the changes in nuclear lamina morphology during the posttranslational processing of preLamin. While farnesylated preLamin can be processed and recovered to its normal shape within 5 – 7 hours, non-farnesylated preLamin remained as aggregates around the nuclear envelope periphery within 24 hours of imaging time course. We believe that our increased understanding of how Lamin undergoes the morphological changes in response to different posttranslational modifications might shed light on the development of new therapeutic drugs for treating lamina-related diseases.

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