The mushroom body (MB) is a prominent central brain structure in most insect. It has been known that a lot of behaviors, the well known is learning and memory, controlled by Drosophila MB. It has been known different Drosophila MB lobes have different functions in memory formation process. Recent studies show some MB connective neurons also play important roles, since they are upstream and downstream of MB, and some of them can modulate MB. Thus, addressing out of a MB extrinsic neurons circuit map is very important how the brain work. However, it is sometimes hard to distinguish from whether those neurons’ neurites are just passing the MB or really innervating the MB under limited resolution microscopes. A novel technique GRASP (GFP reconstitution across synaptic partners), can resolve this problem. This research used GRASP to screen out of MB extrinsic neurons and analyzed their polarities and sectors of fibers that innervate to MB lobes, depict a MB extrinsic neurons sector map, which affording important basic information to understand the MB neuronal circuit, and the brain working model; promoting the future research from lobes to sectors.

蕈狀體是一在大部分的昆蟲中一主要且重要的中樞神經結構。先前研究已知，果蠅蕈狀體控制許多行為，特別是學習與記憶，已熟為人知。先前研究報導，果蠅蕈狀體不同的葉片參與不同形式的記憶形成過程。最近研究也指出一些與果蠅蕈狀體相連的神經細胞也同樣有重要的角色；因為他們是蕈狀體的上下游細胞，且有些可修飾蕈狀體的訊息。故，解出果蠅蕈狀體外來的神經細胞圖譜是項很重要的工作。然而，有時很難辨別這些神經纖維是否有真正深入蕈狀體或只是經過而已。綠色螢光蛋白改編跨越突觸搭檔 (GFP reconstitution across synaptic partners, GFP) 可解決此問題。此研究利用GRASP技術篩選出蕈狀體外來的神經細胞，並分析它們的極性及分佈於蕈狀體葉片所形成的區塊;提供了闡明果蠅蕈狀體神經網路圖譜及大腦運作模型的基礎重要資訊，並促進未來的研究從果蠅蕈狀體葉片的尺度縮小到區塊。

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