應用於厭氧環境下細菌培養之無線傳輸型生物反應器

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研究生：	林鼎舜 Lin, Ding-Shun
論文名稱：	應用於厭氧環境下細菌培養之無線傳輸型生物反應器 Wireless bioreactor for anaerobic cultivation of bacteria
指導教授：	楊雅棠 Yang, Ya-Tang
口試委員:	黃介辰 Huang, Chieh-Chen 張晃猷 Chang, Hwan-you
學位類別：	碩士 Master
系所名稱：	電機資訊學院 - 電子工程研究所 Institute of Electronics Engineering
論文出版年：	2019
畢業學年度：	108
語文別：	中文
論文頁數：	70
中文關鍵詞：	厭氧型細菌、生物反應器、資料無線傳輸、大腸桿菌、氫氣
外文關鍵詞：	anaerobic bacteria, bioreactor, data transmission, Escherichia coli, hydrogen
相關次數：	點閱：2 下載：0
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厭氧型細菌在微生物學裡是一門擁有廣泛興趣的研究，然而其無氧培養技術仍然十分地不便利。此論文裡我們呈現了一項技術可以進行厭氧培養的生物反應器，濁度測量、流體攪拌、氣體傳送以及資料無線傳輸等功能均在封閉的厭氧環境完成。其無氧環境是藉由氣體產氣包而非常見生物實驗室裡的厭氧操作台來達成。此研究以大腸桿菌進行培養並藉由不同碳源和氫氣當作能量來源來呈現。

Anaerobic bacteria are of great interests in wide range of microbiology and yet anaerobic cultivation technique remains cumbersome. Here we report an alternative cultivation strategy based on a bioreactor with the entire operation including turbidity measurement, fluid mixing, gas delivery and data transmission in a gas tight anaerobic enclosure. The anaerobic condition is achieved with gas pack with no need of anaerobic cabinet. Such a cultivation strategy is demonstrated with Escherichia coli with different carbon sources and hydrogen as energy source.

誌謝    I
中文摘要    II
Abstract    III
目錄    IV
圖目錄    VII
表目錄    IX
一、緒論    1
1-1研究動機    1
1-2文獻回顧    3
1-2-1生物反應器的建構    3
1-2-2在不同培養基下的細菌生長    4
二、材料及方法    6
2-1製作厭氧多碳源大腸桿菌樣本    6
2-2藥品配製    7
2-3厭氧環境設計機制    9
2-3-1產氫氣機制    9
2-3-2氧氣排除設計    11
2-4系統設置    11
2-4-1 硬體架設    11
2-4-2光感測生物反應器製作    15
2-4-3自製攪拌系統    17
2-4-4無線傳輸系統    18
2-4-5 泵浦打氣系統    20
2-4-6 Arduino程式編譯    21
2-4校正系統電壓    22
2-5光學量測方法    24
三、實驗結果    25
3-1實驗前準備步驟    25
3-2實驗步驟    26
3-2-1 大腸桿菌在不同碳來源培養基下的厭氧生長    26
3-2-3大腸桿菌在厭氧環境含氫氣環境下的生長    26
3-3培養結果    27
3-3-1 大腸桿菌在不同碳來源培養基下的厭氧生長    27
3-3-2大腸桿菌在厭氧環境含氫氣環境下的生長    29
3-3-3匹配生物反應器實驗    30
四、結論及討論    32
附錄    33
A-1 Arduino 程式碼    33
A-2 生物反應器與壓克架設計圖    42
A-3 大腸桿菌生長速率計算軟體    45
A-4 氫氣與氧氣氣壓測量設置與程式碼    47
A-5 電晶體ST-2L2B 挑選與比較    56
A-6 大腸桿菌厭氧演化實驗    57
參考文獻    58



                                

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