自2006年起，全球各地陸續出現消費者因接觸添加抗生藥劑(Biocides)富馬酸二甲酯(Dimethyl Fumarate)之皮革製品，造成接觸性皮膚炎與急性呼吸疾病等危害，引起社會大眾的恐慌。皮革製品為防止發霉、腐敗，在製造過程中常添加抗生藥劑以維持產品的品質，但無論是製造過程中產生的廢水、廢料排放於環境中，或是直接接觸此類產品，皆使我們暴露於抗生藥劑之風險當中，因此市售皮革製品中的抗生藥劑成分與濃度，值得我們探討。
本研究成功地開發微波輔助萃取技術，萃取皮革製品中富馬酸二甲酯、富馬酸二乙酯、苯並噻唑、對叔丁基苯酚四種抗生藥劑成分，以萃取溶劑甲醇與丙酮混合溶劑（50：50 v/v）、萃取溶劑體積15 mL、微波功率300W、萃取溫度110℃，萃取20分鐘，以中性氧化鋁固相萃取匣淨化的最佳化條件進行萃取，可達到89%以上之回收率，方法偵測極限在0.02~0.06μg/g之間，不僅有良好的回收率且可減少溶劑的用量與萃取的時間。應用於13件市售皮革製品之真實樣品的分析，1件有富馬酸二甲酯的檢出，其濃度為0.5μg/g，超過歐盟所規定之標準0.1 μg/g；13件樣品皆檢出苯並噻唑，濃度在0.04~17.4μg/g之間；8件樣品皆有對叔丁基苯酚的檢出，濃度在0.3~13.0μg/g之間，顯示本研究所開發之微波輔助萃取結合氣相層析質譜儀應用於皮革製品中抗生藥劑成份的分析具有完整確認與可靠檢驗之特點。

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