本研究之目的，在研究多種奈米顆粒及有機小分子作為基因轉植載體對HeLa細胞基因轉植率的影響。第一章簡介文獻上基因治療各種方式及本實驗採用的HeLa cell及pAcGFP及藥物載體等介紹。第二章為儀器及實驗原理介紹，第三章為實驗藥品與儀器設備，第四章則進入實驗中使用到的有機小分子載體的合成過程，第五章E.coli 大量抽取質體步驟，第六、七章則為論文主軸所在，說明各部分實驗的實驗步驟及結果與討論，第八章為結論。
本實驗結果顯示TiO2-20 nm、N doped Fe@CNP、ACh、Nicotine-(CH3)2、pAcGFP此五種藥物在各藥物濃度範圍時(2~500 μg/ml)，細胞存活率皆大於80%；TiO2-300 nm亦有60-70%的細胞存活率，ND-pin、C60-N(CH3)2、Nicotine只有在最高濃度時(500 μg/ml)，細胞存活率才會低於50%，在低濃度範圍，並不會對細胞的生長有太大的影響，而C60-bisadducts和lipofectamine對細胞的毒性較大。在基因轉植的實驗裡，C60-N(CH3)2、C60-bisadducts、ACh此三種藥物的基因轉植率較佳，分別為7.82%、10.38%、8.94%。而TiO2-20 nm在縮短餵藥後細胞再培養的時間後，基因轉植率也從原先的4.71%增加為17.66%；Nicotine- (CH3)2從原先的1.28%增加為7.80%。而從ICP-MS結果可知當餵藥的藥物濃度增加時，單顆細胞攝取藥物的量亦增加。

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