蘑菇型電極量測斑馬成魚與幼魚心電訊號｜國立清華大學博碩士論文庫

簡易檢索 / 詳目顯示

回結果列表

研究生：	施慕蘭 Shih, Mulaine
論文名稱：	蘑菇型電極量測斑馬成魚與幼魚心電訊號 Zebrafish and its Larvae ECG Signal Recording by Mushroom-Shaped Microelectrodes
指導教授：	游萃蓉 Yew, Tri-Rung
口試委員:	彭慧玲張兗君
學位類別：	碩士 Master
系所名稱：	工學院 - 材料科學工程學系 Materials Science and Engineering
論文出版年：	2016
畢業學年度：	104
語文別：	中文
論文頁數：	74
中文關鍵詞：	奈米碳管、微電極、斑馬魚
外文關鍵詞：	CNT, Microelectrode, Zebrafish
相關次數：	點閱：3 下載：0
分享至:	分享至facebook 分享至twitter

查詢本校圖書館目錄查詢臺灣博碩士論文知識加值系統勘誤回報

本研究使用蘑菇形狀金電極及奈米碳管蘑菇形狀金電極來量測斑馬成魚與幼魚的心電訊號，測試其在電生理訊號量測之應用，作為未來研究三維微電極在活體生物病理學研究相關應用的參考。
本研究主要採用奈米碳管低溫製程 (400 °C)，將奈米碳管直接成長於蘑菇形狀金電極上。由於奈米碳管有大表面積、高電容與優良的生物相容性，三維的奈米碳管電極可以改善與待測細胞間的耦合能力和提高訊噪比，因此，奈米碳管蘑菇形狀金電極比蘑菇形狀金電極更具有量測電生理訊號的潛力。此外，本研究利用低溫製程製作的奈米碳管電極，也可用在軟性基板上。
本研究除使用奈米碳管蘑菇形狀金電極、蘑菇形狀金電極量測斑馬成魚離體心臟的心電訊號，並分析不同電極與裝置組合間所量得結果的差異，同時進行藥物的測試，並以等效電路模型進行分析。此外也量測斑馬幼魚心臟的心電訊號，用來展現三維微電極在各年齡斑馬魚之病理學心電量測應用的可能性。

This study is to investigate the detection of rhythmic electrical signals of adult Zebrafish and its larvae using both three dimensional (3D) gold microelectrode and 3D functionalized biocompatible direct-growth carbon nanotube (CNT) microelectrodes. This study also demonstrates the applicability of 3D microelectrode as a tool to study living organisms pathology in the future.
The CNTs were directly grown on 3D gold microelectrodes at 400 °C. With high surface area, capacitance and biocompatibility, 3D funtionalized CNTs improve coupling coefficient and signal-to-noise ratio. Therefore, the 3D CNTs microelectrodes reveal better potential for biosignal recording than 3D gold microelectrodes. In addition, low temperature fabrication process provides the feasibility of implementation with flexible polymer substrates.
The detected adult Zebrafish and its larvae signals were analyzed to compare the results by using different electrodes and system set-ups. The equivalent circuits were also compared for the explanation of measurement results. Furthermore, a preliminary drug test was conducted. Results show the potential application of 3D microelectrodes for all-age Zebrafish pathological Electrocardiogram (ECG) study.

摘要..............................................................................................................I
Abstract......................................................................................................II
誌謝............................................................................................................III
目錄............................................................................................................VI
圖目錄.....................................................................................................VIII
表目錄.......................................................................................................XII
第一章 緒論................................................................................................1
第二章 文獻回顧........................................................................................2
1    微電極 (microelectrode) 在生物電生理量測之應用.......................2
1.1 奈米/微米結構電極在電生理訊號之量測優勢...............................2
1.2 奈米碳管電極發展以及在電生理訊號量測應用............................4
2    電極界面電化學與等效電路分析於生物界面之應用....................5
2.1 常見電化學分析方法在生物體之應用............................................5
2.2 生物應用中使用的電化學結果分析方法與技術簡介....................8
3    斑馬成/幼魚心電訊號之相關研究.................................................10
3.1 應用動物模型研究心電圖簡介......................................................10
3.2 現今應用於斑馬魚心電圖記錄之電極系統比較..........................11
第三章 實驗流程與方法........................................................................14
1    蘑菇形狀電極製備..........................................................................15
1.1 半導體製程技術製備定義圖形試片..............................................15
1.2 硫代硫酸-亞硫酸電鍍法製備蘑菇形狀金電極.............................17
1.3 奈米碳管蘑菇形狀金電極製備......................................................18
2    斑馬成魚量測電極系統架設與定位..............................................21
2.1 固定待測斑馬成魚心臟方法..........................................................21
2.2 電極量測系統架設與電極定位微調操控......................................22
3    斑馬幼魚量測電極系統架設與定位..............................................24
2.1 固定待測斑馬幼魚方法..................................................................24
2.2 電極量測系統架設與電極定位微調操控......................................25
4    儀器簡介..........................................................................................27
4.1 掃描式電子顯微鏡 (Scanning Electron Microscope).....................27
4.2 電化學量測儀器..............................................................................29
4.3 化學氣相沈積高溫爐......................................................................30
4.4 紫外光加臭氧分解機......................................................................31
4.5 電生理量測儀器..............................................................................32
第四章 實驗結果與討論......................................................................33
1    蘑菇形狀電極製程之最佳化..........................................................33
1.1 蘑菇形狀金電極表面形貌與電鍍條件之分析..............................33
1.2 奈米碳管蘑菇形狀金電極表面形貌製程之最佳化......................35
2    蘑菇形狀電極之電化學特性分析..................................................41
2.1 親水處理前後蘑菇形狀電極之電化學特性分析..........................42
2.2 親水處理後蘑菇形狀電極之等效電路分析..................................44
3    斑馬魚量測心電訊號與其等效電路分析......................................48
3.1 心電訊號量測機制之等效電路分析..............................................48
3.2 斑馬成魚心臟訊號量測分析..........................................................50
3.3 斑馬幼魚心臟訊號量測分析..........................................................63

第五章 結論..............................................................................................65
第六章 未來展望......................................................................................66
第七章 參考文獻......................................................................................67
本研究產出之論文發表...........................................................................70

























                                

1. Cogan SF. Neural stimulation and recording electrodes. Annu Rev Biomed Eng. 2008;10:275-309.
2. Chang B-Y, Park S-M. Electrochemical impedance spectroscopy. Annual Review of Analytical Chemistry. 2010;3:207-29.
3. Li J, Ng HT, Cassell A, Fan W, Chen H, Ye Q, et al. Carbon nanotube nanoelectrode array for ultrasensitive DNA detection. Nano letters. 2003;3(5):597-602.
4. Randviir EP, Banks CE. Electrochemical impedance spectroscopy: an overview of bioanalytical applications. Analytical Methods. 2013;5(5):1098-115.
5. Pan AI, Lin M-H, Chung H-W, Chen H, Yeh S-R, Chuang Y-J, et al. Direct-growth carbon nanotubes on 3D structural microelectrodes for electrophysiological recording. Analyst. 2016;141(1):279-84.
6. Fendyur A, Spira ME. Toward on-chip, in-cell recordings from cultured cardiomyocytes by arrays of gold mushroom-shaped microelectrodes. Frontiers in Neuroengineering. 2012;5:21.
7. Hai A, Spira ME. On-chip electroporation, membrane repair dynamics and transient in-cell recordings by arrays of gold mushroom-shaped microelectrodes. Lab on a Chip. 2012;12(16):2865-73.
8. Lieschke GJ, Currie PD. Animal models of human disease: zebrafish swim into view. Nature Reviews Genetics. 2007;8(5):353-67.
9. Liu CC, Li L, Lam YW, Siu CW, Cheng SH. Improvement of surface ECG recording in adult zebrafish reveals that the value of this model exceeds our expectation. Scientific Reports. 2016;6:25073.
10. Milan DJ, Jones IL, Ellinor PT, MacRae CA. In vivo recording of adult zebrafish electrocardiogram and assessment of drug-induced QT prolongation. American Journal of Physiology - Heart and Circulatory Physiology. 2006;291(1):H269-H73.
11. Hassel D, Scholz EP, Trano N, Friedrich O, Just S, Meder B, et al. Deficient zebrafish ether-a-go-go–related gene channel gating causes short-QT syndrome in zebrafish reggae mutants. Circulation. 2008;117(7):866-75.
12. Yu F, Huang J, Adlerz K, Jadvar H, Hamdan MH, Chi N, et al. Evolving cardiac conduction phenotypes in developing zebrafish larvae: implications to drug sensitivity. Zebrafish. 2010;7(4):325-31.
13. Dhillon SS, Dóró É, Magyary I, Egginton S, Sík A, Müller F. Optimisation of embryonic and larval ECG measurement in zebrafish for quantifying the effect of QT prolonging drugs. PloS one. 2013;8(4):e60552.
14. Huang W-C, Hsieh Y-S, Chen IH, Wang C-H, Chang H-W, Yang C-C, et al. Combined use of MS-222 (tricaine) and isoflurane extends anesthesia time and minimizes cardiac rhythm side effects in adult zebrafish. Zebrafish. 2010;7(3):297-304.
15. Zhang X, Tai J, Park J, Tai Y-C, editors. Flexible MEA for adult zebrafish ECG recording covering both ventricle and atrium2014: IEEE.
16. Cao H, Yu F, Zhao Y, Zhang X, Tai J, Lee J, et al. Wearable multi-channel microelectrode membranes for elucidating electrophysiological phenotypes of injured myocardium. Integrative Biology. 2014;6(8):789-95.
17. Zhang X, Beebe T, Jen N, Lee C-A, Tai Y, Hsiai TK. Flexible and waterproof micro-sensors to uncover zebrafish circadian rhythms: The next generation of cardiac monitoring for drug screening. Biosensors and Bioelectronics. 2015;71:150-7.
18. Forouhar AS, Hove JR, Calvert C, Flores J, Jadvar H, Gharib M, editors. Electrocardiographic characterization of embryonic zebrafish2004: IEEE.
19. Rendon-Morales E, Prance RJ, Prance H, Aviles-Espinosa R. Non-invasive electrocardiogram detection of in vivo zebrafish embryos using electric potential sensors. Applied Physics Letters. 2015;107(19):193701.
20. Brüggemann D, Wolfrum B, Maybeck V, Mourzina Y, Jansen M, Offenhäusser A. Nanostructured gold microelectrodes for extracellular recording from electrogenic cells. Nanotechnology. 2011;22(26):265104.
21. Dimaki M, Vazquez P, Olsen MH, Sasso L, Rodriguez-Trujillo R, Vedarethinam I, et al. Fabrication and characterization of 3D micro-and nanoelectrodes for neuron recordings. Sensors. 2010;10(11):10339-55.
22. Gytis B, Emma M, Elisa C, Alberto A, Alberto M, Gian Nicola A, et al. Carbon nanotube composite coating of neural microelectrodes preferentially improves the multiunit signal-to-noise ratio. Journal of Neural Engineering. 2011;8(6):066013.
23. Hai A, Dormann A, Shappir J, Yitzchaik S, Bartic C, Borghs G, et al. Spine-shaped gold protrusions improve the adherence and electrical coupling of neurons with the surface of micro-electronic devices. Journal of The Royal Society Interface. 2009;6(41):1153-65.
24. Hai A, Shappir J, Spira ME. In-cell recordings by extracellular microelectrodes. Nat Meth. 2010;7(3):200-2.
25. Hai A, Shappir J, Spira ME. Long-Term, Multisite, Parallel, In-Cell Recording and Stimulation by an Array of Extracellular Microelectrodes. Journal of Neurophysiology. 2010;104(1):559-68.
26. Hou JH, Kralj JM, Douglass AD, Engert F, Cohen AE. Simultaneous mapping of membrane voltage and calcium in zebrafish heart in vivo reveals chamber-specific developmental transitions in ionic currents. Frontiers in physiology. 2014;5:344.
27. Keefer EW, Botterman BR, Romero MI, Rossi AF, Gross GW. Carbon nanotube coating improves neuronal recordings. Nat Nano. 2008;3(7):434-9.
28. Kim J-H, Kang G, Nam Y, Choi Y-K. Surface-modified microelectrode array with flake nanostructure for neural recording and stimulation. Nanotechnology. 2010;21(8):085303.
29. Lee SM, Byeon HJ, Lee JH, Baek DH, Lee KH, Hong JS, et al. Self-adhesive epidermal carbon nanotube electronics for tether-free long-term continuous recording of biosignals. Scientific Reports. 2014;4:6074.
30. Musameh M, Lawrence NS, Wang J. Electrochemical activation of carbon nanotubes. Electrochemistry Communications. 2005;7(1):14-8.
31. Spira ME, Hai A. Multi-electrode array technologies for neuroscience and cardiology. Nat Nano. 2013;8(2):83-94.
32. Su H-C, Lin C-M, Yen S-J, Chen Y-C, Chen C-H, Yeh S-R, et al. A cone-shaped 3D carbon nanotube probe for neural recording. Biosensors and Bioelectronics. 2010;26(1):220-7.
33. Wang K, Fishman HA, Dai H, Harris JS. Neural stimulation with a carbon nanotube microelectrode array. Nano letters. 2006;6(9):2043-8.
34. Yeh S-R, Chen Y-C, Su H-C, Yew T-R, Kao H-H, Lee Y-T, et al. Interfacing Neurons both Extracellularly and Intracellularly Using Carbon− Nanotube Probes with Long-Term Endurance. Langmuir. 2009;25(13):7718-24.

全文公開日期本全文未授權公開 (校內網路)
全文公開日期本全文未授權公開 (校外網路)
全文公開日期本全文未授權公開 (國家圖書館：臺灣博碩士論文系統)

簡易檢索 / 詳目顯示

相關論文