纖維素藉由葡萄糖單位共價鍵結，構成一長鏈狀之結構，為最豐沛生物聚合物。並且能恣意改質成具有廣泛之化學與機械特性，以及加工成各式形態與形狀。在生醫材料領域上，纖維素和其衍生物良好之生物相容性已經長久地被認可。在這次實驗中，我們研究使用環氧化合物交聯之肝素化纖維素基質(H-CM)作為親和性基質，結合鹼性纖維母細胞生長因子(bFGF)促進細胞增生與基質調控轉基因傳遞之可能性。我們採用人類纖維母細胞(HT-1080)與人類成骨細胞(Saos-2)作為細胞樣本，證實了肝素化纖維素基質(H-CM)相較於未改質過纖維素基質(CM)顯著地改善細胞貼附。細胞ㄧ經貼附，在基質上之細胞便受到來自於鹼性纖維母細胞生長因子(bFGF)之刺激，誘使其提升細胞增生。此外，我們藉由帶負電荷之肝素化纖維素基質(H-CM)吸附陽離子型複合體(PEI/DNA)於基質表面，發現鹼性纖維母細胞生長因子(bFGF)與肝素(heparin)之親和性鍵結會對於陽離子型複合體(PEI/DNA)透過靜電吸引力固定於基質表面造成些許之妨礙。但是，由於鹼性纖維母細胞生長因子(bFGF)將誘導細胞增生，藉此大幅度改善基質調控陽離子型複合體(PEI/DNA)基因轉染效率。最後，我們同時鍵結鹼性纖維母細胞生長因子(bFGF)與吸附含有鹼性纖維母細胞生長因子之陽離子型複合體(PEI/pcDNA3.1(+)-hbFGF)於肝素化纖維素基質(H-CM)，相較於獨自鍵結或吸附有更高之細胞增生速率。我們的研究證明了肝素化纖維素基質(H-CM)為一獨特之材料基質，具有能夠同時調節鹼性纖維母細胞生長因子(bFGF)與含有鹼性纖維母細胞生長因子之陽離子型複合體(PEI/pcDNA3.1(+)-hbFGF)基因傳遞，因此能提供作為組織支架之基質。

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