食物是人類攝取必需微量元素以及有毒元素的主要來源，本研究利用微波消化系統結合感應耦合電漿質譜儀(ICP-MS)應用於稻米中重金屬的總量分析研究，針對多重元素(As, Hg, Pb, Cd, Cu, Cr, Ni, Zn, Se, Al, In, Ga, Mo, W)進行微波消化以及ICP-MS的參數最適化評估。經實驗後選用6 mL conc.HNO3以及3 mL 30%(v/v)H2O2之混合溶液作為最佳消化溶液，於110 ℃的消化溫度下進行消化10分鐘，再根據不同之金屬元素選擇不同之上機模式進行偵測，經NIST標準品進行方法確效得回收率介於80~120 %間，並使用Top-down approach評估此方法之量測不確定度，介於0.08-5.08 mg/kg的範圍內。
本研究收集來自全台75件白米樣品、25種魚類樣品以及42件豬肉樣品，進行現況樣品調查以及我國成年人及國小學童之週/日/月攝入量之評估。由數據結果顯示，75件白米樣品皆符合我國食品藥物管理署(食藥署)針對重金屬鉛、鎘、汞的規範；25種魚類樣品中濃度較高的砷及汞則需進一步建立物種分析方法以確認其中毒性較高之無機砷以及甲基汞的濃度；42件豬肉樣品則是都符合了食藥署針對可食用性家畜類內臟重金屬之規範。攝入量評估方面，我國國小學童各元素之週/日/月攝取量皆符合聯合國糧農組織以及世界衛生組織所制定之規範；成年人則是砷和汞有超標的情形。

Food is the main way of intake of trace elements by human, and rice is the most important food that Taiwanese eat every day. This research aimed to construct a simple, fast and reliable method to analyze multi trace elements in rice by using inductively coupled plasma mass spectrometer, ICP-MS. Prior to instrumentalanalysis, microwave digestion system was successfully applied to decompositionof samples by using 6 ml of concentrated nitric acid and 3 ml of 30 % hydrogen peroxide under 10 minutes and 110 ℃digestion. To validate the proposed method, three Standard Reference Materials from the National Institute of Standard and Technology,were analyzed, and the recovery of each element was in the range of 80-120 %. The measurement uncertainty was alsoevaluated by top-down approach, and the results are in the range of 0.08-5.08 mg/kg.
Afterwards, 75 rice samples from different regions in Taiwan, 25 different species of fish samples and 42 pork samples were collected as field research samples. Finally, the provisional tolerable weekly/daily/monthly intake of adults and elementary childrenwere estimated. All of the 75 rice samples and 42 pork samples are under the regulation for heavy metals set by Food and Drug Administration. Further speciation method is needed for fish samples to check the concentration of inorganic arsenic and methyl mercury. Average weekly/daily/monthly intake of each element of elementary children is under the regulation set by World Health Organization, but the weekly intake of arsenic and mercury of adults are above the regulation.

1. 許正一, 土壤重金屬知多少. 科學發展2011, pp 54-59.
2. 100年我國糧食供需統計結果. 行政院農業委員會2012, (244).
3. Capar, S. G.; Mindak, W. R.; Cheng, J., Analysis of food for toxic elements. Anal Bioanal Chem 2007,389 (1), 159-69.
4. Taylor, A.; Branch, S.; Day, M. P.; Patriarca, M.; White, M., Atomic Spectrometry Update. Clinical and biological materials, foods and beverages. Journal of Analytical Atomic Spectrometry 2009,24 (4), 535-579.
5. Butler, O. T.; Cairns, W. R. L.; Cook, J. M.; Davidson, C. M., Atomic spectrometry update. Environmental analysis. Journal of Analytical Atomic Spectrometry 2010,25 (2), 103-141.
6. 王有忠, 食品安全. 1989, 116-130.
7. 藥物食品安全週報. 2009, (196), p2-3.
8. Krewski, D.; Yokel, R. A.; Nieboer, E.; Borchelt, D.; Cohen, J.; Harry, J.; Kacew, S.; Lindsay, J.; Mahfouz, A. M.; Rondeau, V., Human health risk assessment for aluminium, aluminium oxide, and aluminium hydroxide (vol 10, pg 1, 2008). J. Toxicol. Env. Health-Pt b-Crit. Rev. 2008,11 (2), 147-147.
9. 文永植, 微量元素與人體健康. 國際微量元素醫學會.
10. 王夔, 生命科學中的微量元素第二版. 北京:中國計量出版社1996, p790-814.
11. 徐玉標, 重金屬汙染知多少? 『農業污染』專輯1989, (233).
12. Ambushe, A. A.; McCrindle, R. I.; McCrindle, C. M. E., Speciation of chromium in cow's milk by solid-phase extraction/dynamic reaction cell inductively coupled plasma mass spectrometry( DRC-ICP-MS). Journal of Analytical Atomic Spectrometry 2009,24 (4), 502-507.
13. Bakircioglu, Y.; Bakircioglu, D.; Akman, S., Solid phase extraction of bismuth and chromium by rice husk. J. Trace Microprobe Tech. 2003,21 (3), 467-478.
14. Hagendorfer, H.; Goessler, W., Separation of chromium(III) and chromium(VI) by ion chromatography and an inductively coupled plasma mass spectrometer as element-selective detector. Talanta 2008,76 (3), 656-661.
15. Narin, I.; Kars, A.; Soylak, M., A novel solid phase extraction procedure on Amberlite XAD-1180 for speciation of Cr(III), Cr(VI) and total chromium in environmental and pharmaceutical samples. Journal of hazardous materials 2008,150 (2), 453-458.
16. 施如佳; 陳石松; 張美華; 邱雅琦; 陳怡如; 鄭秋真; 周薰修, 台灣地區貝類重金屬含量分析方法探討及資料之建立. 2006, p325-344.
17. 認識生活環境中毒性物質. 行政院環境保護署2012.
18. Gong, Z. L.; Lu, X. F.; Ma, M. S.; Watt, C.; Le, X. C., Arsenic speciation analysis. Talanta 2002,58 (1), 77-96.
19. 砷、有機砷與無機砷. 藥物食品安全週報2009, (200).
20. 賴文龍, 微量元素對人體之健康. p29-34.
21. Cadmium and cadmium compounds. National Toxicology Program, Department of Health and Huamn Servies 2011.
22. 葉全益, 食品安全學. 華香園出版社2000, p194-206.
23. 廖芳陞, 海水資源:與水汙染相關的疾病. 科學發展2009,438, p26-31.
24. Inorganicand organicleadcompounds. International Agency for Research on Cancer, p39-184.
25. 鍾崇燊; 柯捷男, 可怕的鉛污染. 2002.
26. 林杰樑, 常見重金屬中毒. 綠十字健康網.
27. 許哲綸; 高雅敏; 周秀冠; 鄭守訓; 徐錦豐; 施養志, 食米中重金屬(鎘、汞、鉛)含量之調查. 藥物食品檢驗局調查研究年報2009,27, p205-215.
28. 侯丞; 何啟功, 淺談汞對健康的危害. 1999.
29. 鄭森雄; 許鐘榮, 水俁病. 科學月刊雜誌社1977,87.
30. Commission Regulation (EC) No 1881/2006. Official Journal of the European Union 2006.
31. 食米重金屬限量標準. 衛署食字第 0960406206 號令發布修正.
32. 牛羊豬及家禽可食性內臟重金屬限量標準. 衛署食字第 0930408279 號令發布.
33. Evaluationofcertainfoodadditivesandcontaminants. WHO Technical Report Series.
34. Evaluationofcertainfoodadditivesandcontaminants. WHO Technical Report Series 1982.
35. Evaluationofcertainfoodadditivesandcontaminants-Pb. WHO Technical Report Series 2011,960.
36. Evaluationofcertainfoodadditivesandcontaminants. WHO Technical Report Series 2011,959.
37. Evaluationofcertainfoodadditivesandcontaminants. WHO Technical Report Series 1982,683.
38. 紀柏亨; 楊末雄; 孫毓章, 微波消化之方法應用. 科儀新知1998,56 (4).
39. Estimatingmeasurementuncertaintyinquantitativechemicalanalysis. Federal Agency for the Safety of the Food Chain 2008.
40. 測試結果量測不確定度評估指引. 財團法人全國認證基金會.
41. Nardi, E. P.; Evangelista, F. S.; Tormen, L.; Saint´Pierre, T. D.; Curtius, A. J.; Souza, S. S. d.; Barbosa, F., The use of inductively coupled plasma mass spectrometry (ICP-MS) for the determination of toxic and essential elements in different types of food samples. Food Chemistry 2009,112 (3), 727-732.
42. Shah, A. Q.; Kazi, T. G.; Baig, J. A.; Afridi, H. I.; Arain, M. B., Simultaneously determination of methyl and inorganic mercury in fish species by cold vapour generation atomic absorption spectrometry. Food Chemistry 2012,134 (4), 2345-2349.
43. 國民營養現況:1993~1996國民營養健康狀況變遷調查結果. 行政院衛生署1999.