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研究生: 盧意涵
Lu, Yi-Han
論文名稱: 幼年小鼠社會階級之探討
Study of Social Hierarchy in Young Mice
指導教授: 郭崇涵
Kuo, Tsung-Han
口試委員: 楊世斌
Yang, Shi-Bing
周育如
Chou, Yu-Ju
學位類別: 碩士
Master
系所名稱: 生命科學暨醫學院 - 系統神經科學研究所
Institute of Systems Neuroscience
論文出版年: 2021
畢業學年度: 109
語文別: 英文
論文頁數: 52
中文關鍵詞: 社會階級幼鼠服從者記憶能力
外文關鍵詞: Social hierarchy, Young mice, Subordinate, Memory ability
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  • 社會階級作為一個普遍的社會結構,支持著族群的穩定性以及有效減少成員間的衝突所造成的不必要消耗。目前科學家對於成年時期的社會階級已有一定的了解,但是對於社會階級在幼年時期的研究卻仍未被完整地探討,因此本研究致力於探討幼年期小鼠的社會階級發展及相關的影響因子。利用鑽管測試 (Tube test),我們發現幼年小鼠表現出穩定的社會階級且能保持相同的階級狀況直至成年期;同時我們也意外地發現在鑽管測試中服從者傾向於選擇主動退離衝突環境,而這個選擇並不是受到對於對手的認知或是感官訊息的影響,而是服從者的自我認知影響了它在衝突中所選擇的決策。另一方面,探討社會階級相關影響因子的實驗並沒有顯示壓力及憂鬱、焦慮行為在支配者與服從者間有差異,但是在記憶能力方面,支配者在物體認知及空間記憶能力皆優於服從者,遺憾的是,我們未能成功了解記憶及社會階級之間的因果關係。未來我們將繼續探討服從者自我認知的形成機制及記憶與社會階級間的交互作用,並期望本研究能提供新的觀點去了解社會階級在幼年時期形成的意義及相關形成機制。


    Social hierarchy, as a universal social structure, maintains the stability of the group and reduces unnecessary conflicts between members. While current research has mostly focused on the studies of adult social hierarchy, social status in young animals was rarely explored. In this study, we aim to evaluate a social hierarchy in young mice and explore the related characteristics. In tube test, weanling mice showed a stable social hierarchy, which could consistently continue into adulthood. In addition, we unexpectedly observed that the conflict was not resolved by dominant but by subordinate mice, who were tended to voluntarily withdraw in tube test. This decision was mainly affected by internal status rather than recognition or social sensory signals from opponents. In addition, we also investigated different factors potentially involved in social hierarchy, and found dominants showed a significantly better ability of recognition and spatial working memory than subordinates. The relationship among stress, anxiety, depressive behavior and social hierarchy were not detected. Unfortunately, our experimental designs fail to clarify the cause and effect between memory and social hierarchy. In the future, we will continue to explore the mechanism of internal status in subordinate mice and the connection between memory and social hierarchy, and hope this research can provide new perspectives of the formation of social hierarchy in young animals

    1.Introduction...7 1.1 Introduction of social hierarchy...7 1.2 Social hierarchy in species...7 1.3 Social hierarchy in mice...8 1.4 The studies of social behavior in youngsters...8 1.5 Characteristics related to social hierarchy...9 1.6 Research aim...10 2. Materials and Methods...11 2.1. Animals...11 2.2. Non-training tube test...11 2.3 Platform competition...12 2.4. Drug administration...12 2.5. Blocker assay...12 2.6. Mouse restrain...13 2.7. Y maze forced alternative test...13 2.8. Novel object recognition test...14 2.9. Social hierarchy switching...14 2.10. Open field test...14 2.11. Light dark box test...15 2.12. Forced swimming test...15 2.13. Tail suspension test...16 2.14. Data Analysis...16 2.14.1. Stability and hierarchy relationship...16 2.14.2. Tube test Parameters...17 2.14.3. Statistics...17 3. Results...18 3.1. Weanling mice perform stable social hierarchy...18 3.2. The correlation of social hierarchy between weanling mice and adult mice...18 3.3. Subordinate mice voluntarily retreat in tube test...19 3.4. Subordination is driven by internal status...19 3.5. The level of stress, anxiety and depression were not related to social hierarchy...21 3.6. Dominant mice showed better recognition memory ability...22 4. Discussion...24 4.1. Weanling mice performed stable and sustainable social hierarchy...24 4.2. The decisive role of subordinates in social hierarchy...25 4.3. Acute stress did not affect the social hierarchy in weanling mice...26 4.4. Level of depression and anxiety did not relate to social hierarchy in weanling mice...27 4.5. Dominant mice showed better memory ability than subordinate mice...27 4.6. Conclusion...28 5. Reference...30 6. Figure...32

    Absher, John R., and Jasmin Cloutier. Neuroimaging Personality, Social Cognition, and Character. 2016
    Angel, Ariel, RotemVolkman, Tabitha GraceRoyal, andDanielOffen. 2020. “Caspase-6 Knockout in the 5xFAD Model of Alzheimer’s Disease Reveals Favorable Outcome on Memory and Neurological Hallmarks.” International Journal of Molecular Sciences 21 (3). https://doi.org/10.3390/ijms21031144.
    Barnard, C. J., andN.Luo. 2002. “Acquisition of Dominance Status Affects Maze Learning in Mice.” Behavioural Processes 60 (1): 53–59. https://doi.org/10.1016/S0376-6357(02)00121-3.
    Brothers, Denis J., andCharles D.Michener. 1974. “Interactions in Colonies of Primitively Social Bees - III. Ethometry of Division of Labor in Lasioglossum Zephyrum (Hymenoptera: Halictidae).” Journal of Comparative Physiology 90 (2): 129–68. https://doi.org/10.1007/BF00694482.
    Charafeddine, Rawan, HugoMercier, FabriceClément, LaurenceKaufmann, AnneReboul, andJean BaptisteVan derHenst. 2016. “Children’s Allocation of Resources in Social Dominance Situations.” Developmental Psychology 52 (11): 1843–57. https://doi.org/10.1037/dev0000164.
    Chu, Xixia, YingZhou, ZhiqiangHu, JingyuLou, WeiSong, JingLi, XiaoLiang, et al. 2016. “24-Hour-Restraint Stress Induces Long-Term Depressive-Like Phenotypes in Mice.” Scientific Reports 6 (September): 1–12. https://doi.org/10.1038/srep32935.
    Earth, The. 1971. “Mammals Mark When Dominant to and Intolerant” 285 (February).
    Fan, Zhengxiao, HongZhu, TingtingZhou, ShengWang, YanWu, andHailanHu. 2019. “Using the Tube Test to Measure Social Hierarchy in Mice.” Nature Protocols 14 (3): 819–31. https://doi.org/10.1038/s41596-018-0116-4.
    Kraus, Michael W., Jun WonPark, andJacinth J.X.Tan. 2017. Signs of Social Class: The Experience of Economic Inequality in Everyday Life. Perspectives on Psychological Science. Vol. 12. https://doi.org/10.1177/1745691616673192.
    Langley, Ellis J.G., Jayden O.VanHorik, Mark A.Whiteside, andJoah R.Madden. 2018. “Group Social Rank Is Associated with Performance on a Spatial Learning Task.” Royal Society Open Science 5 (2): 1–9. https://doi.org/10.1098/rsos.171475.
    Martin, S. J., andR. G.M.Morris. 2002. “New Life in an Old Idea: The Synaptic Plasticity and Memory Hypothesis Revisited.” Hippocampus 12 (5): 609–36. https://doi.org/10.1002/hipo.10107.
    Mitchell, Nathan C., Georgianna G.Gould, Corey M.Smolik, WouterKoek, andLynette C.Daws. 2013. “Antidepressant-like Drug Effects in Juvenile and Adolescent Mice in the Tail Suspension Test: Relationship with Hippocampal Serotonin and Norepinephrine Transporter Expression and Function.” Frontiers in Pharmacology 4 (OCT): 1–9. https://doi.org/10.3389/fphar.2013.00131.
    Nyby, John, Gerard A.Dizinno, andGlaydeWhitney. 1976. “Social Status and Ultrasonic Vocalizations of Male Mice.” Behavioral Biology 18 (2): 285–89. https://doi.org/10.1016/S0091-6773(76)92198-2.
    Papale, Ligia A., AndyMadrid, SisiLi, andReid S.Alisch. 2017. “Early-Life Stress Links 5-Hydroxymethylcytosine to Anxiety-Related Behaviors.” Epigenetics 12 (4): 264–76. https://doi.org/10.1080/15592294.2017.1285986.
    Park, Min Jung, Bo AmSeo, BoyoungLee, Hee SupShin, andMyoung GooKang. 2018. “Stress-Induced Changes in Social Dominance Are Scaled by AMPA-Type Glutamate Receptor Phosphorylation in the Medial Prefrontal Cortex.” Scientific Reports 8 (1): 1–14. https://doi.org/10.1038/s41598-018-33410-1.
    Roseth, Cary J., Anthony D.Pellegrini, Danielle N.Dupuis, Catherine M.Bohn, Meghan C.Hickey, Caroline L.Hilk, andAnniePeshkam. 2011. “Preschoolers’ Bistrategic Resource Control, Reconciliation, and Peer Regard.” Social Development 20 (1): 185–211. https://doi.org/10.1111/j.1467-9507.2010.00579.x.
    Smith, Brian H. 1987. “Hierarchy in the Primitively Eusocial Bee Lasioglossum Zephyrum.” Animal Behaviour 35: 211–17.
    Wang, Fei, JunZhu, HongZhu, QiZhang, ZhanminLin, andHailanHu. 2011. “Bidirectional Control of Social Hierarchy by Synaptic Efficacy in Medial Prefrontal Cortex.” Science 334 (6056): 693–97. https://doi.org/10.1126/science.1209951.

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