近年來、基因療法 ( Gene therapy ) 用於治療癌症，已成為一種具展望性的治療策略於對抗肝癌 ( HCC ; hepatocellular carcinoma )。然而治療性核酸質體( Plasmid DNA ) 能否成功運輸至細胞內並調控癌細胞表現，是眾多研究所面臨的一大挑戰。因此在本研究中，我們利用已知對 HCC 具有標靶作用的 SP94 胜肽修飾脂質磷酸鈣奈米載體 ( Liposome Calcium Phosphate Nanoparticle ，LCPP-NPs ) 標靶肝癌細胞。藉由具有核定位訊號 ( Nuclear Localization Signal ， NLS ) 魚精蛋白 ( Protamine ) 以提高轉染效果，讓癌細胞高度表達特定蛋白表現。更進一步，我們使用奈米粒子搭載具有誘導細胞凋亡的基因片段-腫瘤壞死因子相關凋亡誘導配體- TRAIL( Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand )，專一性的運輸至肝癌細胞中進行基因治療，希望能藉此讓肝癌細胞進行細胞凋亡，達到治療癌症效果。總結來說，這個具腫瘤標靶控細胞基因表現，達到顯著的基因治療目的。

Gene therapy for cancer treatment has become the very promising approach of hepatocellular carcinoma (HCC). However, the therapeutic plasmid DNA delivery into cancer cell and regulate the expression is a challenge of many studies. We utilize SP94 peptide as targeted ligand modifies liposome calcium phosphate nanoparticles (LCP-NPs) to specifically target HCC. We also use protamine to improve transfection efficiency which contains several nuclear localization signal (NLS). Moreover, we want to deliver an apoptosis inducer gene -TRAIL (Tumor necrosis factor-related apoptosis-inducing ligand) to induce tumor cell apoptosis. These results demonstrated that targeting LCP-NPs can specifically delivery therapeutic plasmid DNA into HCC to regulate cancer cell expression.

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