本研究為三維果蠅實驗平台之溫度控制與導入系統之自動化，其內容分兩部分:溫度控制系統及自動化導入平台設計-含機構設計與自動化系統控制。本研究之第一部分主要探討果蠅之熱刺激環境的溫度場，採用中空玻璃球作為實驗環境，命名為生物球，熱源以數個薄型加熱片貼附於玻璃表面，並根據模擬結果假設實驗控制之加熱環境。環境監控平台設有八個溫度讀取點與四個加熱片，加熱片之溫度控制結果於40℃下之安定時間約4分鐘並可維持半小時之穩定狀態，最終穩態誤差小於1%。第二部分主要建立一自動化導入平台，透過自動化機構簡化果蠅實驗前之準備工作，以取代一部分人力，導入平台原型由壓克力與ABS塑膠製成，控制物件包括DC馬達、伺服馬達等，平台測試重點分有前段(刺激與計數)、後段(平移與導入)，測試結果，計數正確率為81%、後段導入通過率為95%，整體實驗流程約5~10分鐘，可提供15隻果蠅/次導入至生物球。

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